APC Antibody

• 产品编号：CST-2504S      品牌：CST       原厂货号：2504S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

APC抗体检测内源性的总APC蛋白。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源APC蛋白序列一致，抗体由蛋白A和肽段亲和层析技术纯化。腺瘤结肠息肉（APC）肿瘤抑制基因在大部分家族性和散发性大肠癌中突变，编码一个大的胞质蛋白，与细胞迁移，粘附和增殖有关（1）。APC直接结合微管，APC的缺失会因为染色体错误分离导致有丝分裂纺锤体缺陷和非整倍体产生（2）。APC被认为有支架蛋白特征，结合β-catenin，涉及胞内浓度调控。Wnt信号不存在时，GSK-3β磷酸化APC-β-catenin-axin的复合物的三成员，β-catenin的磷酸化创出一个泛素识别位点，这是一个蛋白酶调节降解的信号。Wnt存在时，dishevelled使GSK-3β失活，β-catenin协调对细胞周期进程和增殖中重要蛋白基因的转录，如cyclin D1和 c-Myc（3）。

1. Shih, I. M. et al. (2000) Cancer Res. 60, 1671-1676.
2. Kaplan, K. B. et al. (2001) Nat Cell Biol. 3, 429-432.
3. Fodde, R. (2002) Eur J Cancer. 38, 86-871.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

APC Antibody detects endogenous levels of total APC protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human APC. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Adenomatous Polyposis Coli (APC) tumor suppressor gene is mutated in most familial and sporadic colorectal cancers and encodes a large cytoplasmic protein that is implicated in cell migration, cell adhesion, and proliferation (1). APC binds directly to microtubules and lack of APC leads to defective mitotic spindles and aneuploidy due to missegregation of chromosomes (2). APC is well characterized as a scaffolding protein, binds to β-catenin, and is involved in the regulation of its intracellular concentration. In the absence of a Wnt signal, GSK-3β phosphorylates all three members of the APC-β-catenin-axin complex and this phosphorylation of β-catenin creates a recognition site for ubiquitin, the signal for proteasome-mediated degradation. In the presence of a Wnt signal, dishevelled inactivates GSK-3β and β-catenin coordinates gene transcription of proteins important for the control of cell cycle progression and proliferation, such as cyclin D1 and c-Myc (3).

1. Shih, I. M. et al. (2000) Cancer Res. 60, 1671-1676.
2. Kaplan, K. B. et al. (2001) Nat Cell Biol. 3, 429-432.
3. Fodde, R. (2002) Eur J Cancer. 38, 86-871.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody