eIF4A1 Antibody

• 产品编号：CST-2490S      品牌：CST       原厂货号：2490S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

eIF4A1 Antibody检测内源性eIF4A1总蛋白水平。通过人工合成人源eIF4A1蛋白Gly12位点周围序列相应的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

众多细胞因子参与了蛋白质翻译起始阶段重要的生物过程。翻译起始因子eIF4F复合物结合到5'-端m7GTP帽子结构从而解开mRNA的二级结构，以使小核糖体的附着(1)。 eIF4A作为eIF4复合物的组成成分，其是ATP依赖性RNA解旋酶。该蛋白能够解开mRNA5’端非翻译区的二级结构，以便核糖体的结合(2,3)。此外，最近研究显示在果蝇中eIF4A以一种独立于翻译过程的方式抑制Dpp/BMP信号(4,5)。

1. Rogers, G.W. et al. (2001) J. Biol. Chem. 276, 12598-12608.
2. Rogers, G.W. et al. (1999) J. Biol. Chem. 274, 12236-12244.
3. Svitkin, Y.V. et al. (2001) RNA 7, 382-394.
4. Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414.
5. Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321.

原厂资料：

Specificity / Sensitivity

eIF4A1 Antibody detects endogenous levels of total eIF4A1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Gly12 of human eIF4A1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

A variety of factors contribute to the important biological event of initiation of translation. The eIF4F complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signalling in a translation-independent manner in Drosophila (4,5).

1. Rogers, G.W. et al. (2001) J. Biol. Chem. 276, 12598-12608.
2. Rogers, G.W. et al. (1999) J. Biol. Chem. 274, 12236-12244.
3. Svitkin, Y.V. et al. (2001) RNA 7, 382-394.
4. Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414.
5. Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody