eIF5 Antibody

• 产品编号：CST-2480S      品牌：CST       原厂货号：2480S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

eIF5 Antibody检测内源性eIF5总蛋白水平。通过人工合成人源eIF5蛋白Val417位点周围序列相应多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

通过人工合成人源eIF5蛋白Val417位点周围序列相应多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

1. Das, S. and Maitra, U. (2001) Prog Nucleic Acid Res Mol Biol 70, 207-31.
2. Chakrabarti, A. and Maitra, U. (1991) J Biol Chem 266, 14039-45.
3. Asano, K. et al. (2000) Genes Dev 14, 2534-46.
4. Das, S. et al. (2001) J Biol Chem 276, 6720-6.
5. Paulin, F.E. et al. (2001) Curr Biol 11, 55-9.

原厂资料：

Specificity / Sensitivity

eIF5 Antibody detects endogenous levels of total eIF5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence surrounding Val417 of human eIF5. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Eukaryotic translation initiation factor 5 (eIF5) is crucial for the assembly of translation initiation complex and plays an important role in protein synthesis (1). eIF5 interacts with the 43S initiation complex to stimulate hydrolysis of GTP bound to eIF2 (1-3). Studies suggest that eIF5 functions as the GTPase-activating protein (GAP) in the hydrolysis of GTP-bound eIF2 (4,5). This hydrolysis leads to the release of initiation factors from the 40S ribosomal subunit, which is a necessary step in the formation of the 80S initiation complex (1).

1. Das, S. and Maitra, U. (2001) Prog Nucleic Acid Res Mol Biol 70, 207-31.
2. Chakrabarti, A. and Maitra, U. (1991) J Biol Chem 266, 14039-45.
3. Asano, K. et al. (2000) Genes Dev 14, 2534-46.
4. Das, S. et al. (2001) J Biol Chem 276, 6720-6.
5. Paulin, F.E. et al. (2001) Curr Biol 11, 55-9.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.