Phospho-VEGF Receptor 2 (Tyr996) Antibody

• 产品编号：CST-2474S      品牌：CST       原厂货号：2474S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化VEGF Receptor 2 (Tyr996) Antibody 仅在Tyr 996磷酸化时才能检测内源VEGFR2 蛋白的水平。本抗体与酪氨酸磷酸化PDGF receptor交叉反应。多克隆抗体用合成的磷酸化多肽免疫动物得到，该多肽根据人VEGF receptor 2 的Tyr996附近的氨基酸合成。抗体经过protein A和亲和层析纯化。血管内皮生长因子受体2 (VEGFR2, KDR, Flk-1)是内皮细胞上VEGF诱导信号时的一个主要受体。和配体结合之后，VEGFR2发生自磷酸化并被活化(1)。VEGFR2 主要的自磷酸化受体位于激酶插入区 (Tyr951/996)和酪氨酸激酶催化区(Tyr1054/1059) (2)。受体的激活会导致对包括Shc, Grb2, PI3K激酶, Nck 和蛋白酪氨酸磷酸化酶SHP-1 和 SHP-2接头蛋白的快速招募(3)。Tyr1212的磷酸化为Grb2提供了结合位点而且磷酸化的Tyr1175能结合PI3K激酶的p85 亚基以及PLCγ和 Shb (1,4,5)。VEGFR2信号传导对于实现VEGF刺激引起的增殖、趋化和发芽，以及体外培养的内皮细胞的增殖和体内血管的发生是必需的(6-8)。

1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

原厂资料：

Specificity / Sensitivity

Phospho-VEGF Receptor 2 (Tyr996) Antibody detects endogenous VEGFR 2 only when phosphorylated at tyrosine 996. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr996 of human VEGF receptor 2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.