Glu-Glu Tag Antibody用来检测包含有Glu-Glu表位标签的转染蛋白。此抗体可以识别哺乳动物中一氨基或羧基末端融合到目的蛋白的Glu-Glu标签。多克隆抗体是采用合成肽段(蓝色载体结合)免疫动物生产的,抗体采用蛋白A和肽亲和色谱法纯化。在免疫印迹、免疫沉淀和免疫染色等技术中,表位标签用于标记和检测蛋白。由于它们的体积很小,它们一般不会影响标记蛋白的生化特性。
原厂资料:
Specificity / Sensitivity
Glu-Glu Tag Antibody detects transfected proteins containing the Glu-Glu epitope tag. The antibody recognizes the Glu-Glu tag fused to either the amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
注意事项:
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.