ATGL (30A4) Rabbit mAb

• 产品编号：CST-2439S      品牌：CST       原厂货号：2439S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ATGL (30A4)兔单克隆抗体识别内源性的ATGL总蛋白水平。该单克隆抗体用与小鼠ATGL蛋白中Pro469位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。

甘油三酯在许多生物体形成重要的能量储存。在哺乳动物中脂肪组织作为主要甘油三酯的储存部位。脂肪分解酶动员甘油三酯在饥饿期间为生物体提供必要的能量。激素敏感脂肪酶（HSL）是首个发现的脂肪分解酶，在哺乳动物中水解脂肪组织中的甘油三酯（1-3）。其它脂肪分解酶，包括脂肪甘油三酯脂肪酶（ATGL），也被发现。ATGL的主要功能是催化脂质分子的酯键水解。这种酶可以为HSL水解提供甘油二酯底物。ATGL在小鼠的白色和棕色脂肪组织中大量表达，具有高度的底物特异性（4）。ATGL被独立确定为desnutrin（5）和甘油水解酶诱导的磷脂酶A2ζ（6）。

1. Holm, C. et al. (1988) Science 241, 1503-1506.
2. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
3. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
4. Zimmermann, R. et al. (2004) Science 306, 1383-1386.
5. Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
6. Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.

原厂资料：

Specificity / Sensitivity

ATGL (30A4) Rabbit mAb detects endogenous levels of total ATGL protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro469 of mouse ATGL.

Background

Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).

1. Holm, C. et al. (1988) Science 241, 1503-1506.
2. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87, 533-537.
3. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273, 215-221.
4. Zimmermann, R. et al. (2004) Science 306, 1383-1386.
5. Villena, J.A. et al. (2004) J. Biol. Chem. 279, 47066-47075.
6. Jenkins, C.M. et al. (2004) J. Biol. Chem. 279, 48968-48975.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.