Phospho-Shc (Tyr239/240) Antibody

• 产品编号：CST-2434P      品牌：Cell Signaling Technology       原厂货号：2434P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Shc (Tyr239/240) Antibody能检测酪氨酸（239/240位点）磷酸化的Shc 蛋白水平。该抗体可能与活化的EGF受体蛋白发生交叉反应。该多克隆抗体通过使用与人源Shc蛋白酪氨酸（239/240位点）周围残基相一致的合成磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。Shc包含SH2和PTB结构域，并且是各种受体酪氨酸激酶信号转导过程中的骨架蛋白。Shc以p46, p52，p66异构体的形式存在，这是使用选择性转位抑制位点、不同剪接信息的结果(1-3)。应答细胞内信号，Shc蛋白的SH2和PTB结构域与活化的受体相互作用，导致Shc蛋白三个不同酪氨酸（239/240/317位点)的磷酸化(4-6)。GRB2/Sos蛋白结合酪氨酸（239/240/317位点)磷酸化的Shc蛋白，激活Ras/Raf/MAPK通路(4)。Shc蛋白的表达以及它的酪氨酸位点磷酸化在胸腺T细胞的发育中起着重要且不可或缺的作用(7)。

1. Pelicci, G. et al. (1992) Cell 70, 93-104.
2. Migliaccio, E. et al. (1997) EMBO J. 16, 706-716.
3. Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049.
4. Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836.
5. Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204.
6. Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444.
7. Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755.

原厂资料：

Specificity / Sensitivity

Phospho-Shc (Tyr239/240) Antibody detects Shc only when phosphorylated at tyrosine 239/240. The antibody may cross-react with activated EGF receptor protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).

1. Pelicci, G. et al. (1992) Cell 70, 93-104.
2. Migliaccio, E. et al. (1997) EMBO J. 16, 706-716.
3. Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049.
4. Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836.
5. Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204.
6. Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444.
7. Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.