Calnexin Antibody

• 产品编号：CST-2433S      品牌：CST       原厂货号：2433S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Calnexin Antibody识别内源性的calnexin总蛋白。该多克隆抗体用与人类calnexin蛋白Ala51位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。分泌型和跨膜型蛋白以多核糖体形式合成，然后转移到内质网中进行常见的二硫键形成、氨基糖基化和折叠。为了帮助蛋白质正确折叠，内质网包含一些列分子伴侣，包括calnexin。Calnexin首先被作为鼠I类组织相容性分子而发现（1，2）。Calnexin是嵌入内质网膜的钙结合蛋白，可以将新合成的糖蛋白保留在内质网内，确保其正确折叠和进行质量控制（3-5）。Calnexin对一组糖蛋白的特异性通过瘦素位点界定，它结合折叠的糖蛋白上的一种早期寡糖中间体（5）。

1. Degen, E. and Williams, D.B. (1991) J. Cell Biol. 112, 1099-1115.
2. Ahluwalia, N. et al. (1992) J. Biol. Chem. 267, 10914-10918.
3. Rajagopalan, S. et al. (1994) Science 263, 387-390.
4. Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128.
5. Williams, D.B. (2006) J. Cell Sci. 119, 615-623.

原厂资料：

Specificity / Sensitivity

Calnexin Antibody detects endogenous levels of total calnexin protein.

Specificity / Sensitivity

Calnexin Antibody detects endogenous levels of total calnexin protein.

Background

Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. Calnexin was first identified as being involved in the assembly of murine class I histocompatibility molecules (1,2). Calnexin is a calcium-binding protein embedded in the ER membrane that retains the newly synthesized glycoproteins inside the ER to ensure proper folding and quality control (3-5). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (5).

1. Degen, E. and Williams, D.B. (1991) J. Cell Biol. 112, 1099-1115.
2. Ahluwalia, N. et al. (1992) J. Biol. Chem. 267, 10914-10918.
3. Rajagopalan, S. et al. (1994) Science 263, 387-390.
4. Bergeron, J.J. et al. (1994) Trends Biochem. Sci. 19, 124-128.
5. Williams, D.B. (2006) J. Cell Sci. 119, 615-623.

注意事项：

Storage: Supplied in 10mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.