PPARγ (D69) Antibody

• 产品编号：CST-2430S      品牌：CST       原厂货号：2430S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PPARγ (D69) Antibody能够检测内源性水平的总PPARγ蛋白。PPARγ (D69) Antibody 通过用合成肽免疫动物制备，该合成肽是人PPARγ天冬氨酸（69位）附近的残基。过氧化物酶体增殖物激活受体γ（PPARγ）是配体激活的核受体超家族成员，作为转录激活剂起作用（1）。 PPARγ优先在脂肪细胞、血管平滑肌细胞和巨噬细胞表达（2）。除此之外，它在介导脂肪细胞分化和脂质代谢中起作用（2），PPARγ调节胰岛素敏感性、细胞增殖和炎症（3）。 PPARγ的转录活性可以被MAP激酶通过磷酸化PPARγ丝氨酸（84位）抑制（4,5）。

1. Tontonoz, P. et al. (1995) Curr. Opin. Genet. Dev. 5, 571-576.
2. Rosen, E.D. et al. (1999) Mol. Cell 4, 611-617.
3. Murphy, G.J. and Holder, J.C. (2000) Trends Pharmacol. Sci. 21, 469-474.
4. Camp, H.S. and Tafuri, S.R. (1997) J. Biol. Chem. 272, 10811-10816.
5. Adams, M. et al. (1997) J. Biol. Chem. 272, 5128-5132.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

PPARγ (D69) Antibody detects endogenous levels of total PPARγ protein.

Source / Purification

PPARγ (D69) Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp69 of human PPARγ.

Background

Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5).

1. Tontonoz, P. et al. (1995) Curr. Opin. Genet. Dev. 5, 571-576.
2. Rosen, E.D. et al. (1999) Mol. Cell 4, 611-617.
3. Murphy, G.J. and Holder, J.C. (2000) Trends Pharmacol. Sci. 21, 469-474.
4. Camp, H.S. and Tafuri, S.R. (1997) J. Biol. Chem. 272, 10811-10816.
5. Adams, M. et al. (1997) J. Biol. Chem. 272, 5128-5132.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.