p14 ARF (4C6/4) Mouse mAb

• 产品编号：CST-2407S      品牌：CST       原厂货号：2407S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

 p14 ARF (4C6/4) Mouse mAb能够检测内源性p14 ARF 总蛋白。该单克隆抗体是由重组的带有His标签的p14 ARF 蛋白免疫动物生产的。人类p14 ARF (小鼠中为p19 ARF)是一种促凋亡的细胞周期调节子，在人类肿瘤中经常性失活(1)。在大多数细胞中p14 ARF的基础表达水平是比较低的，但是当癌基因表达时会发生该蛋白的累积(2,3)。表达增加的p14 ARF促进MDM2的降解，导致p53蛋白水平的升高和随后的细胞周期捕获和(或)细胞凋亡(4)。尽管大多数p14 ARF信号传统意义上被认为是p53依赖的，但新近的报道表明p53非依赖的p14 ARF信号也引起细胞周期捕获和细胞凋亡(5,6)。

1. Eischen, C. M. et al. (1999) Genes Dev. 13, 2658-2669.
2. de Stanchina, E. et al. (1998) Genes Dev. 12, 2434-2442.
3. Zindy, F. et al. (1998) Genes Dev. 12, 2424-2433.
4. Pomerantz, J. et al. (1998) Cell 92, 713-723.
5. Weber, J. D. et al. (2000) Genes Dev. 14, 2358-2365.
6. Hemmati, P. G. et al. (2002) Oncogene 21, 3149-3161.

原厂资料：

Specificity / Sensitivity

p14 ARF (4C6/4) Mouse mAb detects endogenous levels of total p14 ARF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant His-tagged p14 ARF protein.

Background

Human p14 ARF (p19 ARF in mouse) is a pro-apoptotic cell cycle regulator frequently inactive in human tumors (1). Basal expression of p14 ARF is low in most cell types, but accumulation of this protein occurs in response to oncogene expression (2,3). Increased p14 ARF levels facilitate MDM2 degradation, leading to increased p53 protein levels and subsequent cell cycle arrest and/or apoptosis (4). While most p14 ARF signaling has traditionally thought to be p53-dependent, more recent reports have described p53-independent p14 ARF signaling leading to cell cycle arrest and apoptosis (5,6). 

1. Eischen, C. M. et al. (1999) Genes Dev. 13, 2658-2669.
2. de Stanchina, E. et al. (1998) Genes Dev. 12, 2434-2442.
3. Zindy, F. et al. (1998) Genes Dev. 12, 2424-2433.
4. Pomerantz, J. et al. (1998) Cell 92, 713-723.
5. Weber, J. D. et al. (2000) Genes Dev. 14, 2358-2365.
6. Hemmati, P. G. et al. (2002) Oncogene 21, 3149-3161.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium
azide. Store at –20°C. Do not aliquot the antibody.