Phospho-HSP27 (Ser15) Antibody

• 产品编号：CST-2404S      品牌：CST       原厂货号：2404S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-HSP27 (Ser15) Antibody识别内源性的Ser15磷酸化的HSP27蛋白。该多克隆抗体用与人类HSP27中Ser15位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

热休克蛋白（HSP）27是小HSP之一，在多种细胞和组织中都有不同水平的组成性表达。像其它小HSP一样，HSP27的调节也发生在转录和翻译后（1）。应激反应中，HSP27表达增加了数倍以实现细胞对不利环境的抵抗。HSP27被MAPKAPK-2在Ser15、Ser78和Ser82磷酸化，作为p38 MAP激酶途径活化的结果（2,3）。HSP27磷酸化导致其四级结构改变，以至于从大分子量的同型多聚体变为二聚体和单体（4）。目前显示HSP27磷酸化和浓度增加可以调节肌动蛋白多聚化和再组织（5,6）。

1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

原厂资料：

Specificity / Sensitivity

Phospho-HSP27 (Ser15) Antibody detects endogenous levels of HSP27 only when phosphorylated at serine 15.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser15 of human HSP27. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody