Ribosomal protein L7a Antibody检测内源性核糖体蛋白L7a的总蛋白。使用该抗体进行免疫荧光染色显示核糖体蛋白L7a主要定位在内质网膜和核仁，分别是翻译和核糖体合成位置。这显示它可以作为核糖体的一个标记。通过人工合成人源ribosomal protein L7a蛋白Arg225位点周围相应的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Ribosomal protein L7a是一个高度保守的核糖体蛋白定位于60S核糖体亚基(1)。该蛋白含有不同的功能域，其作用是将新合成的多肽运送到细胞核和核仁，核仁是核糖体生物合成位置(2)。Ribosomal protein L7a也能在体外通过该蛋白质的两个不同的RNA结合区域与RNA相互作用(3)。综上，核仁的定位和结合RNA能力显示ribosomal protein L7a可能作为核糖体的生物合成和行使功能的重要组分。
Ribosomal protein L7a Antibody detects endogenous levels of total ribosomal protein L7a protein. Immunofluorescent staining using this antibody shows ribosomal protein L7a localized mostly on the ER membrane and in nucleoli, sites for translation and ribosome synthesis, respectively, indicating its utility as a ribosomal marker.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg225 of human ribosomal protein L7a. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Ribosomal protein L7a is a highly conserved ribosome protein localized to 60S ribosomal subunit (1). The protein has distinct domains that target the newly synthesized polypeptide to nucleus and the nucleoli, the site of ribosome biosynthesis (2). Ribosomal protein L7a can also interact with RNA in vitro through two distinct RNA-binding domains in the protein (3). Taken together, nucleolar localization and the ability to bind RNA suggests that ribosomal protein L7a may act as an important component for ribosome biosynthesis and function.
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