p38 MAPK (D13E1) XP? Rabbit mAb (Biotinylated)

• 产品编号：CST-2387S      品牌：CST       原厂货号：2387S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

p38 MAPK (D13E1) XP® Rabbit mAb (Biotinylated) recognizes endogenous levels of total p38α, β, or γ MAPK protein. This antibody does not recognize p38δ, SAPK/JNK, or p44/42 MAPK proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p38 MAPK protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated p38 MAPK (D13E1) XP® Rabbit mAb #8690.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

p38 MAPK (D13E1) XP® Rabbit mAb (Biotinylated) recognizes endogenous levels of total p38α, β, or γ MAPK protein. This antibody does not recognize p38δ, SAPK/JNK, or p44/42 MAPK proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p38 MAPK protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated p38 MAPK (D13E1) XP® Rabbit mAb #8690.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). 
 SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10)

1. Rouse, J. et al. (1994) Cell 78, 1027-37.
2. Han, J. et al. (1994) Science 265, 808-11.
3. Lee, J.C. et al. (1994) Nature 372, 739-46.
4. Freshney, N.W. et al. (1994) Cell 78, 1039-49.
5. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.
6. Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.
7. Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.
8. Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.
9. Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
10. Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody.