Itk (2F12) Mouse mAb

• 产品编号：CST-2380S      品牌：CST       原厂货号：2380S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Itk (2F12) Mouse mAB 能够检测内源性Itk蛋白总体水平。此单克隆抗体是通过合成人源对应的Itk N-末端周围的肽段来免疫动物而获得。

白介素-2 诱导T-细胞激酶(Itk, Emt or Tsk) 是无受体蛋白酪氨酸激酶成员之一。Itk家族成员包括Tec, Btk, Rlk 和 Bmx。它们通过共有的结构来定义:N-末端的PH域, 一个Tec同源域，一个SH3和SH2域以及紧随其后的C-末端激酶域(1)。Tec, Rlk 和 Itk都在T细胞中表达并且通过响应T细胞受体(TCR)的招募而激活。已有数据表明Itk在TCR下游的信号转导中起作用并激活PLCgamma1和Erk。Lck通过磷酸化Itk中保守的活性环中 Tyr511位点而将其激活, 此外，Itk还能在SH3域的 Tyr180位点自磷酸化。与失去功能的Itk-Y511F相比，Itk-Y180F仍然能够磷酸化PLCgamma1(2-3)。Itk -/- 小鼠表现出肺部炎症的减少，噬曙红细胞渗透降低和响应过敏性哮喘诱导中粘液产生也减少。因此，Itk 可能成为平喘药的有效靶点(4)。

1. Schwartzberg, P.L. and Finkelstein, L.D. (2005) Nat Rev Immunol. 5, 284-295.
2. Heyeck, S. D. et al. (1997) J Biol Chem. 272, 25401-25408.
3. Wilcox, H.M. and Berg, L.J. (2003) J Biol Chem. 278, 37112-37121.
4. Mueller, C. and August, A. (2003) J Immunol. 170, 5056-5063.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Itk (2F12) Mouse mAB detects endogenouse levels of total Itk protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with an amino-terminal peptide of Itk.

Background

Interleukin-2 inducible T-cell kinase (Itk, Emt or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain and a SH3 and SH2 domain followed by a carboxy-terminal kinase domain (1). Tec, Rlk and Itk are expressed in T cells and activated in response to T cell receptor (TCR) engagement. Data demonstrate that Itk functions in signal transduction downstream of TCR and activates PLCgamma1 and Erk. Lck directly activates Itk through phosphorylation in the conserved activation loop at Tyr511, and furthermore, Itk is autophosphorylated in the SH3 domain at Tyr180. Itk-Y180F is still capable of phosphorylating PLCgamma1 in contrast to Itk-Y511F, which has lost that function (2-3). Itk -/- mice show reduced lung inflammation, eosinophil infiltration and mucous production in response to allergic asthma induction. Thus, Itk could become a desirable target for anti-asthmatic treatments (4).

1. Schwartzberg, P.L. and Finkelstein, L.D. (2005) Nat Rev Immunol. 5, 284-295.
2. Heyeck, S. D. et al. (1997) J Biol Chem. 272, 25401-25408.
3. Wilcox, H.M. and Berg, L.J. (2003) J Biol Chem. 278, 37112-37121.
4. Mueller, C. and August, A. (2003) J Immunol. 170, 5056-5063.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.