Dynamin I/II Antibody

• 产品编号：CST-2342S      品牌：CST       原厂货号：2342S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Dynamin I/II Antibody识别内源性的dynamin总蛋白。此抗体不识别相关的dynamin类似的蛋白DLP。该多克隆抗体用与人类dynamin蛋白中Tyr231位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。Dynamin是大GTP酶的一类家族，可能与内吞或分泌过程中的囊泡形成有关（1）。Dynamin在细胞表面受体内化中扮演重要作用，受体内化可以减弱细胞对细胞外信号的反应。Dynamin被显示可以与信号蛋白如Src、PLCγ、PKC和G蛋白等信号蛋白相互作用。PKC和Src磷酸化Dynamin，它的磷酸化可以调节细胞表面受体的内吞（2，3）。

1. McNiven, M.A. et al. (2000) Trends Biochem. Sci. 25, 115-120.
2. Powell, K.A. et al. (2000) J. Biol. Chem. 275, 11610-11617.
3. Ahn, S. et al. (1999) J. Biol. Chem. 274, 1185-1188.

原厂资料：

Specificity / Sensitivity

Dynamin I/II Antibody detects endogenous levels of total dynamin protein. The antibody does not recognize the related dynamin-like protein DLP.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr231 of human dynamin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both the endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCγ, PKC and G-proteins. PKC and Src phosphorylate dynamin, and its phosphorylation may regulate the endocytosis of cell surface receptors (2,3).

1. McNiven, M.A. et al. (2000) Trends Biochem. Sci. 25, 115-120.
2. Powell, K.A. et al. (2000) J. Biol. Chem. 275, 11610-11617.
3. Ahn, S. et al. (1999) J. Biol. Chem. 274, 1185-1188.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.