Phospho-SirT1 (Ser27) Antibody

• 产品编号：CST-2327S      品牌：CST       原厂货号：2327S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

该抗体检测仅在serine 27位点磷酸化的内源性SirT1蛋白。该抗体不能与其它sirtuin蛋白发生交叉反应。通过合成的与人源SirT1蛋白Ser27位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Silent Information Regulator (SIR2)家族基因是高度保守基因组，它们能编码烟酰胺腺呤二核苷酸依赖的蛋白去乙酰化酶，又称为组蛋白去乙酰化酶(histone deacetylase,HDAC)3。这些基因的最初发现和最好的特征是酿酒酵母( Saccharomyces cerevisiae)SIR2，它涉及交配型位点、端粒维持、DNA损伤反应和细胞老化的沉默(1)。SIRT1是哺乳动物Sir2的直系同源物，它是一个细胞核蛋白并且涉及调节许多细胞内过程，包括凋亡、细胞衰老、内分泌信号、糖稳态、老龄化和长寿 。SirT1的靶蛋白包含acetylated p53 (2,3)、p300 (4)、Ku70 (5)、forkhead (FoxO) transcription factors (5,6)、PPARγ (7)和PPARγ coactivator-1α (PGC-1α)蛋白(8)。p53和FoxO转录因子的去乙酰化作用可以抑制凋亡和增加细胞生存(2,3,5,6)。PPARγ和PGC-1α蛋白的去乙酰化作用可以调节在肝脏中糖异生/糖酵解通路和在空腹条件下白色脂肪细胞中脂肪的动态(7,8)。 SirT1去乙酰酶的活性是通过烟酰胺抑制和通过白藜芦醇激活。此外，SirT1蛋白活性可能是通过磷酸化作用调节，因为在体外Ser27和Ser47位点被磷酸化，然而，这些磷酸化位点的功能还没有被确定(9)。

1. Guarente, L. (1999) Nat. Genet. 23, 281-285.
2. Vaziri, H. et al. (2001) Cell 107, 149-159.
3. Luo, J. et al. (2001) Cell 107, 137-148.
4. Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
5. Brunet, A. et al. (2004) Science 303, 2011-2015.
6. Motta, M.C. et al. (2004) Cell 116, 551-563.
7. Picard, F. et al. (2004) Nature 429, 771-776.
8. Rodgers, J.T. et al. (2005) Nature 434, 113-118.
9. Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.

原厂资料：

Specificity / Sensitivity

This antibody detects endogenous levels of SirT1 protein only when phosphoryated on serine 27. The antibody does not cross-react with other sirtuin proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser27 of human SirT1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7) and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9).

1. Guarente, L. (1999) Nat. Genet. 23, 281-285.
2. Vaziri, H. et al. (2001) Cell 107, 149-159.
3. Luo, J. et al. (2001) Cell 107, 137-148.
4. Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
5. Brunet, A. et al. (2004) Science 303, 2011-2015.
6. Motta, M.C. et al. (2004) Cell 116, 551-563.
7. Picard, F. et al. (2004) Nature 429, 771-776.
8. Rodgers, J.T. et al. (2005) Nature 434, 113-118.
9. Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.