Phospho-Synapsin (Ser9) Antibody

• 产品编号：CST-2311S      品牌：CST       原厂货号：2311S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Synapsin (Ser9) Antibody 兔多抗识别内源性的Ser9磷酸化的synapsin蛋白。该抗体可以识别所有Ser9磷酸化的synapsin亚型。该多克隆抗体由合成的人类synapsin I蛋白Ser9位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Synapsins是一组至少包括五个相关成员（Synapsins IA，IB，IIA，IIB，和IIIa）的蛋白，在脑内丰富，在调节神经递质的释放中必不可少（1,2）。所有的synapsins都包含一个短的氨基末端结构域，它高度保守，可被PKA或钙调蛋白激酶I磷酸化（1）。synapsin氨基末端域Ser9的磷酸化会抑制其与磷脂的结合，将其从突触囊泡上分解出来（2）。

1. Greengard, P. (1987) Mol Neurobiol 1, 81-119.
2. Hosaka, M. et al. (1999) Neuron 24, 377-87.

原厂资料：

Specificity / Sensitivity

Phospho-Synapsin (Ser9) Antibody detects endogenous levels of synapsin only when phosphorylated at serine 9. The antibody is expected to recognize phosphorylated serine 9 of all synapsin isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 9 of human synapsin I. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).

1. Greengard, P. (1987) Mol Neurobiol 1, 81-119.
2. Hosaka, M. et al. (1999) Neuron 24, 377-87.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.