Aven Antibody

• 产品编号：CST-2300S      品牌：CST       原厂货号：2300S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Aven Antibody 能够检测内源Aven蛋白总体水平。多克隆抗体是通过合成Aven对应的C-末端的多肽来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。

Bcl-2家族蛋白通过控制线粒体中细胞色素C的释放和caspase-9的激活，从而对大多数的刺激作出响应，并控制凋亡过程(1-3)。在细胞色素C与dATP存在下，凋亡因子Apaf-1与caspase-9形成复合体，激活caspase-9，紧接着激活caspase-3。很多蛋白都能与Bcl-2蛋白及其它帮助调控凋亡的家族成员蛋白互作。在酵母双杂交筛选中，Aven被鉴定为 bcl-xL 互作蛋白 (4)。它也能与其它的抗凋亡家族成员作用，包括Bcl-2, 但是却不能与促凋亡蛋白Bax和Bak互作。Aven抑制凋亡并且增加抗凋亡因子Bcl-xL的活性。它能干扰与Apaf-1相关的蛋白并激活caspase-9。Aven 的过表达与急性白血病的不良预后相关(5,6)。

1. Harris, M.H. and Thompson, C.B. (2000) Cell Death. Differ. 7, 1182-1191.
2. Cory, S. et al. (2003) Oncogene 22, 8590-8607.
3. Scorrano, L. and Korsmeyer, S.J. (2003) Biochem. Biophys. Res. Commun. 304, 437-444.
4. Chau, B.N. et al. (2000) Mol. Cell 6, 31-40.
5. Choi, J. et al. (2006) Leuk. Res. 30, 1019-1025.
6. Paydas, S. et al. (2003) Ann. Oncol. 14, 1045-1050.

原厂资料：

Specificity / Sensitivity

Aven Antibody detects endogenous levels of total Aven protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of Aven. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Bcl-2 family regulates apoptosis in response to a wide range of stimuli through control of mitochondrial cytochrome c release and caspase activation (1-3). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3. A large number of proteins have been found to interact with Bcl-2 and other family members that have been shown to help regulate apoptosis. Aven was identified in a yeast two-hybrid screen as a bcl-xL interacting protein (4). It also interacts with other anti-apoptotic family members, including Bcl-2, but fails to interact with pro-apopotic proteins Bax and Bak. Aven inhibits apoptosis and enhances anti-apopotic activity of Bcl-xL. It interferes with association with Apaf-1 and activation of caspase-9. Aven overexpression is associated with poor prognosis in acute lymphoblastic leukemia (5,6).

1. Harris, M.H. and Thompson, C.B. (2000) Cell Death. Differ. 7, 1182-1191.
2. Cory, S. et al. (2003) Oncogene 22, 8590-8607.
3. Scorrano, L. and Korsmeyer, S.J. (2003) Biochem. Biophys. Res. Commun. 304, 437-444.
4. Chau, B.N. et al. (2000) Mol. Cell 6, 31-40.
5. Choi, J. et al. (2006) Leuk. Res. 30, 1019-1025.
6. Paydas, S. et al. (2003) Ann. Oncol. 14, 1045-1050.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.