DRAK2 (33D7) Rabbit mAb

• 产品编号：CST-2294S      品牌：CST       原厂货号：2294S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DRAK2 (33D7) Rabbit mAb兔单抗能够检测内源性水平DRAK2总蛋白。该单克隆抗体是采用合成的人源DRAK2蛋白天冬氨酸(315位)周围残基相对应的肽段免疫动物而制备的。

DRAK2（DAP激酶相关凋亡诱导蛋白激酶2）是 DAP（死亡相关蛋白）的促凋亡蛋白激酶家族成员（1）。DRAK2在NIH/3T3细胞中的过表达，会诱导细胞发生相关的形态学变化及细胞凋亡，这可能是以p53依赖的方式发生的（1,2）。DRAK2在淋巴组织中优先表达，在胸腺细胞选择性进程中调节TCR的激活阈值（3）。事实上，来自DRAK2(-/-)小鼠的T细胞对T细胞受体介导的刺激表现出增强敏感性，并减少对协同刺激的需求（4）。

1. Kögel, D. et al. (2001) Bioessays 23, 352-358.
2. Sanjo, H. et al. (1998) J. Biol. Chem. 273, 29066-29071.
3. Friedrich, M.L. et al. (2005) Int. Immunol. 17, 1379-1390.
4. McGargill, M.A. et al. (2004) Immunity 21, 781-791.

原厂资料：

Specificity / Sensitivity

DRAK2 (33D7) Rabbit mAb detects endogenous levels of total DRAK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surroundingAsp315 of mouse DRAK2.

Background

DRAK2 (DAP kinase related apoptosis inducing protein kinase 2) is a member of the novel DAP (death associated protein) pro-apoptotic kinase family (1). Overexpression of DRAK2 in NIH/3T3 cells induces morphological changes associated with apoptosis, which are likely to occur in a p53-dependent manner (1,2). DRAK2 is preferentially expressed in lymphoid tissues and regulates the TCR activation threshold during thymocyte selection (3). Indeed, T cells from DRAK2(-/-) mice exhibit enhanced sensitivity to T cell receptor-mediated stimulation and have a reduced requirement for co-stimulation (4).

1. Kögel, D. et al. (2001) Bioessays 23, 352-358.
2. Sanjo, H. et al. (1998) J. Biol. Chem. 273, 29066-29071.
3. Friedrich, M.L. et al. (2005) Int. Immunol. 17, 1379-1390.
4. McGargill, M.A. et al. (2004) Immunity 21, 781-791.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.