Shh Antibody

• 产品编号：CST-2287S      品牌：CST       原厂货号：2287S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Shh抗体识别转染水平的Shh蛋白的前体和成熟体形态。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Shh蛋白His35氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化。Hedgehog蛋白（Hh）为分泌信号蛋白，在动物发育中起多种作用，不正常的Hh信号活性可能与众多的出生缺陷有关，失控的Hh通路激活与一些癌症发展有关（1-2）。三种确定的脊椎动物Hh基因是Sonic (Shh), Indian (Ihh)和 Desert (Dhh),所有这些都有各自独特作用，也有重叠作用（3-5）。Hh蛋白被合成为45kDa前体，经过自剪切来产生19kDa氨基末端（Hh-N）和一个C末端肽段（Hh-C）。这氨基末端肽段的C末端变成共价结合于一个胆固醇分子，其N末端乙酰化，这双重修饰Hh-N肽段从细胞释放后参与了已知的所有Hedgehog信号活化过程（6）。

1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
3. Zhang, X.M. et al. (2001) Cell 106, 781-792.
4. Adolphe, C. et al. (2004) Development 131, 5009-5019.
5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Dog

Specificity / Sensitivity

Shh antibody detects both the precursor and mature forms of transfected Shh.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His35 of human Shh. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
3. Zhang, X.M. et al. (2001) Cell 106, 781-792.
4. Adolphe, C. et al. (2004) Development 131, 5009-5019.
5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody