DAP1 Antibody

• 产品编号：CST-2282S      品牌：CST       原厂货号：2282S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

能够检测内源性水平DAP1总蛋白。该抗体不会与DAP产生交叉反应。该多克隆抗体是采用合成的人源DAP1蛋白94位氨基酸周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

死亡相关蛋白1（DAP1）是一个15 kDa的蛋白，是作为一个γ-干扰素起始的细胞死亡正调节子（1，2）。DAP1蛋白富含脯氨酸，并拥有一个SH3结合基序，以及一些普遍蛋白激酶磷酸化位点（1）。该蛋白定位在细胞质中，但其凋亡功能的详细机制目前还不清楚。死亡相关蛋白3 （DAP3）广泛表达，在膜受体介导的细胞凋亡过程中表达上调。在γ-干扰素和Fas诱导的细胞凋亡中，DAP3作为一个正介体，作用于下游的受体信号复合物和上游的效应器半胱天冬酶（3,4）。死亡相关蛋白5 （DAP5）是一个97 kDa蛋白，具有与真核翻译起始因子4G（Elf4G）高度同源的氨基酸序列（1,5）。与elF4G相比，DAP5缺乏cap-依赖性翻译所需的氨基末端区域，作为γ-干扰素诱导的细胞死亡的调节子，具有独特的羧基末端区（5,6）。在细胞凋亡诱导期间，DAP5在天冬氨酸790位被切割。羧基端截短形式的DAP5作为一个帽独立翻译起始因子，负责在细胞凋亡过程中翻译的调解（7）。

1. Deiss, L.P. et al. (1995) Genes Dev 9, 15-30.
2. Levy-Strumpf, N. and Kimchi, A. (1998) Oncogene 17, 3331-40.
3. Kissil, J.L. et al. (1995) J Biol Chem 270, 27932-6.
4. Kissil, J.L. et al. (1999) EMBO J 18, 353-62.
5. Imataka, H. et al. (1997) EMBO J 16, 817-25.
6. Levy-Strumpf, N. et al. (1997) Mol Cell Biol 17, 1615-25.
7. Henis-Korenblit, S. et al. (2000) Mol Cell Biol 20, 496-506.

原厂资料：

Specificity / Sensitivity

DAP1 Antibody detects endogenous levels of total DAP1 protein. It does not cross-react with other DAP family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 94 of human DAP1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Death associated protein 1 (DAP1) is a 15 kDa protein that functions as a positive mediator of cell death initiated by interferon-gamma (1, 2). The DAP1 protein is proline rich and possesses one SH3 binding motif, as well as several consensus protein kinase phosphorylation sites (1). The protein is localized in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear. Death associated protein 3 (DAP3) is widely expressed, and the expression is upregulated during membrane receptor-mediated apoptosis. In interferon-gamma- and Fas-induced apoptosis, DAP3 acts as a positive mediator, functioning downstream of the receptor signaling complex and upstream of the effector caspases (3,4). Death associated protein 5 (DAP5) is a 97 kDa protein with a high degree of amino acid sequence homology to eukaryotic translation initiation factor 4G (Elf4G) (1,5). Compared with elF4G, DAP5 lacks the amino-terminal region necessary for cap-dependent translation, and has a unique carboxy-terminal region that functions as a regulator of interferon-gamma-induced cell death (5,6). During induction of apoptosis, DAP5 is cleaved at aspartic acid 790. The carboxy-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis (7).

1. Deiss, L.P. et al. (1995) Genes Dev 9, 15-30.
2. Levy-Strumpf, N. and Kimchi, A. (1998) Oncogene 17, 3331-40.
3. Kissil, J.L. et al. (1995) J Biol Chem 270, 27932-6.
4. Kissil, J.L. et al. (1999) EMBO J 18, 353-62.
5. Imataka, H. et al. (1997) EMBO J 16, 817-25.
6. Levy-Strumpf, N. et al. (1997) Mol Cell Biol 17, 1615-25.
7. Henis-Korenblit, S. et al. (2000) Mol Cell Biol 20, 496-506.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.