SPAK Antibody

• 产品编号：CST-2281S      品牌：CST       原厂货号：2281S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SPAK Antibody检测内源性SPAK总蛋白。该抗体不能与OSR1蛋白或GCK家族的其它成员发生相互作用。通过人工合成人源SPAK蛋白Ser436位点周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化蛋白。SPAK (STE20/SPS1-related Pro/Ala-rich kinase)和OSR1 (oxidative stress responsive 1)是GCK丝氨酸/苏氨酸激酶家族成员。过表达和体外研究证明SPAK蛋白能够激活p38 MAP激酶，其表明在应激反应下SPAK蛋白的一个可能的角色(1)。酵母双杂交试验揭示SPAK和OSR1蛋白结合到Na-K-2Cl转运蛋白NKCC1和NKCC2以及K-Cl转运蛋白KCC3 (2)。WNK1和WNK4激酶使SPAK蛋白在Thr243/247和Ser380位点发生磷酸化(3-5)。同样地，WNK1和WNK4激酶使OSR1蛋白在Thr185和Ser315位点发生磷酸化(3, 4)。在这些位点的磷酸化刺激SPAK和OSR1的激活，这导致NKCC1的磷酸化和提高NKCC1的活性(3-5)。在T细胞的激活反应下，通过PKCθ激酶使SPAK蛋白也在Ser311位点磷酸化。Ser311被Ala替换或SPAK蛋白特异siRNA干扰显著性降低TCR/CD28诱导AP-1激活，这认为SPAK也涉及T细胞信号(6)。

1. Johnston, A.M. et al. (2000) Oncogene 19, 4290-7.
2. Piechotta, K. et al. (2002) J Biol Chem 277, 50812-9.
3. Vitari, A.C. et al. (2005) Biochem J 391, 17-24.
4. Moriguchi, T. et al. (2005) J Biol Chem 280, 42685-93.
5. Gagnon, K.B. et al. (2006) Mol Cell Biol 26, 689-98.
6. Li, Y. et al. (2004) EMBO J 23, 1112-22.

原厂资料：

Specificity / Sensitivity

SPAK Antibody detects endogenous levels of total SPAK protein. This antibody does not cross-react with OSR1 or other members of the GCK family.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser436 of human SPAK. Antibodies are purified by protein A and peptide affinity chromatography.

Background

SPAK (STE20/SPS1-related Pro/Ala-rich kinase) and OSR1 (oxidative stress responsive 1) are members of the GCK family serine/threonine kinases. Overexpression and in vitro studies demonstrate that SPAK is able to activate p38 MAP kinase indicating a possible role for SPAK in the stress response (1). Yeast two-hybrid screening revealed that SPAK and OSR1 bind to Na-K-2Cl cotransporters NKCC1 and NKCC2 and K-Cl cotransporter KCC3 (2). WNK1 and WNK4 phosphorylate SPAK at Thr243/247 and Ser380 (3-5). Similarly, WNK1 and WNK4 phosphorylate OSR1 at Thr185 and Ser315 (3, 4). Phosphorylation at these sites stimulates SPAK and OSR1 activity, leading to NKCC1 phosphorylation and enhanced NKCC1 activity (3-5). SPAK is also phosphorylated at Ser311 by PKCθ in response to T cell activation. Substitution of Ser311 with Ala or specific siRNA knock-down of SPAK dramatically reduces TCR/CD28-induced AP-1 activation, suggesting SPAK is involved in T cell signaling as well (6).

1. Johnston, A.M. et al. (2000) Oncogene 19, 4290-7.
2. Piechotta, K. et al. (2002) J Biol Chem 277, 50812-9.
3. Vitari, A.C. et al. (2005) Biochem J 391, 17-24.
4. Moriguchi, T. et al. (2005) J Biol Chem 280, 42685-93.
5. Gagnon, K.B. et al. (2006) Mol Cell Biol 26, 689-98.
6. Li, Y. et al. (2004) EMBO J 23, 1112-22.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody