Myc-Tag (9B11) Mouse mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-2279S      品牌：CST       原厂货号：2279S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Myc-Tag (9B11) Mouse mAb (Alexa Fluor ® 488 Conjugate)可以检测细胞中包含Myc标为标签的蛋白。此抗体可以识别转染细胞靶蛋白在氨基或羧基末端融合的Myc标签 。单克隆抗体是采用与人类c-Myc (EQKLISEEDL)的410-419残基相对应的合成肽段免疫动物而生产的。抗体在最佳条件下与Alexa Fluor ® 555结合，F/P为2-6.此种抗体 在最佳条件下采用Alexa Fluor ® 488荧光染料标记，已经过内部测试，可以直接用于Myc标签蛋白转染细胞的流式细胞术和免疫荧光分析。在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。Myc标为标签在细菌、酵母和哺乳动物细胞系统中广泛应用于检测重组蛋白的表达。

1. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

原厂资料：

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 488 Conjugate) detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL). The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in cells transfected with Myc-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

1. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in PBS (pH 7.2), less than 0.1% Sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.