MMP-9 (G657) Antibody

• 产品编号：CST-2270S      品牌：CST       原厂货号：2270S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MMP-9 (G657)抗体可以识别全长（酶原，92 kDa）人和小鼠的MMP-9。合成对应人MMP-9657位甘氨酸及其邻近氨基酸残基序列的多肽序列免疫动物获得多克隆抗体。抗体由蛋白A和肽亲和层析纯化。

基质金属蛋白酶（MMP）属于蛋白酶家族能够识别许多细胞外基质蛋白包括其他蛋白酶，生长因子，细胞表面受体和黏附分子（1）。在这些家族成员中，认为MMP-2, MMP-3, MMP-7 和 MMP-9是正常组织重塑过程中的重要因子，这些过程包括胚胎发育，损伤修复，癌症侵润，血管新生，致癌作用和细胞凋亡（2-4）。MMP活性与癌症发展相关联（2）。MMP调控的一个机制需要蛋白水解从全长蛋白上释放抑制性前肽结构域以形成有活性的MMPs（5）。

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
4. Vu, T.H. et al. (1998) Cell 93, 411-22.
5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

原厂资料：

Specificity / Sensitivity

MMP-9 (G657) Antibody detects full length (proenzyme, 92 kDa) human and mouse MMP-9.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence based around amino acid residue Gly657 of human MMP-9. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
4. Vu, T.H. et al. (1998) Cell 93, 411-22.
5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.