Evi-1 Antibody

• 产品编号：CST-2265S      品牌：CST       原厂货号：2265S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Evi1抗体能够检测内源Evi-1蛋白总体水平和MDS1/Evi蛋白。此多克隆抗体是通过合成人源对应的Evi-1 N-末端 Leu921位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Evi-1 (热带病毒整合位点1)最初是从小鼠髓系白血病中病毒的普遍整合位点鉴定而来。它通过染色体的易位和倒置而参与了骨髓相关疾病(1)并在实体肿瘤的形成中发挥作用(2)。Evi-1是一个锌指转录因子，在动物的发育过程中起了重要作用(3)。由于利用不同的5‘端 (4)可变剪切(5)使其有多重形式，可变剪切导致了在正常组织中产生与MDS1融合的蛋白(6)。

1. Nucifora, G. et al. (2006) Gene 368, 1-11.
2. Brooks, D.J. et al. (1996) Br. J. Cancer 74, 1518-25.
3. Hoyt, P.R. et al. (1997) Mech. Dev. 65, 55-70.
4. Aytekin, M. et al. (2005) Gene 356, 160-8.
5. Morishita, K. et al. (1990) Oncogene 5, 963-71.
6. Fears, S. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 1642-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

Evi1 Antibody detects endogenous levels of total Evi-1 and MDS1/Evi proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Leu921 of human Evi-1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Evi-1 (Ecotropic virus integration site 1) was originally identified as a common site of viral integration in murine myeloid leukemia. It is involved in human myeloid disorders through chromosome translocation and inversion (1) and is also implicated in solid tumor formation (2). Evi-1 is a zinc finger transcription factor which also plays an important role in animal development (3). It has many isoforms due to alternative usage of 5'-ends (4), alternative splicing (5), and intergenic splicing which results in the formation of a fusion protein with MDS1 in normal tissues (6).

1. Nucifora, G. et al. (2006) Gene 368, 1-11.
2. Brooks, D.J. et al. (1996) Br. J. Cancer 74, 1518-25.
3. Hoyt, P.R. et al. (1997) Mech. Dev. 65, 55-70.
4. Aytekin, M. et al. (2005) Gene 356, 160-8.
5. Morishita, K. et al. (1990) Oncogene 5, 963-71.
6. Fears, S. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 1642-7.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.