Phospho-(Ser) PKC Substrate Antibody

• 产品编号：CST-2261S      品牌：CST       原厂货号：2261S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-(Ser) PKC Substrate Antibody可识别内源性在+2和-2位上有精氨酸或赖氨酸，并且+1位是疏水氨基酸的磷酸化丝氨酸的细胞内蛋白质。该抗体不与非磷酸化丝氨酸残基、同样基序中磷酸化的苏氨酸残基、或其它基序中的磷酸化丝氨酸残基发生交叉反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及相应国外专利）该多克隆抗体用合成的磷酸化PKC底物肽段免疫动物制备。该抗体使用蛋白A和多肽亲和层析纯化。

尽管蛋白激酶C（PKC）家族成员参与多种信号转导过程，包括分泌、基因表达、增殖和肌肉收缩（1,2），但它们的很多底物仍然未确定。PKC的同工酶分为传统型（cPKC），新型PKC（nPKC）和非典型PKC（aPKC）。PKCα, βI, βII, 和γ属于cPKC组（1）。激活后cPKC同工酶磷酸化精氨酸或赖氨酸在-3,-2和+2位，和+1位是疏水氨基酸的丝氨酸和苏氨酸（1-3）。为了鉴别潜在的PKC底物，Cell Signaling Technology公司开发了识别此基序的抗体。Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb兔单抗特异性识别包含磷酸化丝氨酸的cPKC底物。

1. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
3. Obata, T. et al. (2000) J Biol Chem 275, 36108-15.

原厂资料：

Specificity / Sensitivity

Phospho-(Ser) PKC Substrate Antibody detects endogenous levels of many cellular proteins only when phosphorylated at serine residues surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. The antibody does not cross-react with nonphosphorylated serine residues, with phospho-threonine in the same motif, or with phospho-serine in other motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho-PKC substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.

Background

Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser.

1. Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60.
2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
3. Obata, T. et al. (2000) J Biol Chem 275, 36108-15.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.