PRMT5/Skb1Hs Methyltransferase Antibody

• 产品编号：CST-2252S      品牌：CST       原厂货号：2252S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PRMT5/Skb1Hs Methyltransferase Antibody能够检测内源性Skb1Hs总的甲基转移酶蛋白水平。该抗体不能与其它相关的甲基转移酶发生相互作用。通过合成的与人源Skb1Hs甲基转移酶氨基酸100周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

人源Skb1Hs methyltransferase(也称为JBP1)是一个酵母蛋白Skb1和Hsl7p的同系物 (1,2)，它有637个氨基酸组成，并且包含蛋白甲基转移酶中保守的结构域。在体外它能使组蛋白和MBP发生甲基化(2)。酵母Hsl7p是通过负性调节Swe1p蛋白涉及调节细胞周期G2进程，而Swe1p蛋白是一个蛋白质酪氨酸激酶，它可磷酸化和抑制Cdc28p蛋白(3)。一个Hsl7p同系物Skb1在分裂期酵母中凭借酵母双杂交与Shk1p相互作用从而被鉴定，Shk1p是一个p21 (cdc42p/Rac) activated kinase (PAK) (4)。两个蛋白都属于蛋白质甲基转移酶亚家族(5)。有趣地，人源Skb1Hs甲基转移酶证明与Jak激酶相互作用。这认为的可能性是在哺乳细胞中Skb1Hs甲基转移酶能联系Jak到一个PAK信号通路。

1. Gilbreth, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14781-14786.
2. Pollack, B.P. et al. (1999) J. Biol. Chem. 274, 31531-31542.
3. Ma, X.J. et al. (1996) Genes Dev. 10, 1327-1340.
4. Gilbreth, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 13802-13807.
5. Ma, X.J. et al. (2000) Trends Biochem. Sci. 25, 11-12.

原厂资料：

Specificity / Sensitivity

PRMT5/Skb1Hs Methyltransferase Antibody detects endogenous levels of total Skb1Hs methyltransferase protein. The antibody does not cross-react with other related mammalian methyltransferases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 100 of human Skb1Hs methyltransferase. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases. It methylates histones and MBP in vitro (2). Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3). An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast two-hybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4). Both proteins belong to the protein methyltransferase superfamily (5). Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases. This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.

1. Gilbreth, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14781-14786.
2. Pollack, B.P. et al. (1999) J. Biol. Chem. 274, 31531-31542.
3. Ma, X.J. et al. (1996) Genes Dev. 10, 1327-1340.
4. Gilbreth, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 13802-13807.
5. Ma, X.J. et al. (2000) Trends Biochem. Sci. 25, 11-12.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody