PU.1 (9G7) Rabbit mAb (Alexa Fluor? 647 Conjugate)

• 产品编号：CST-2240S      品牌：CST       原厂货号：2240S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

PU.1 (9G7) Rabbit mAb (Alexa Fluor® 647 Conjugate)能够检测内源PU.1蛋白总体水平。此抗体不与Ets家族蛋白的其它成员交叉反应。此单克隆抗体是通过合成对应人源性PU.1序列的肽段免疫动物而获得。此抗体在F/P 比例为 2-6的最优化条件下与 Alexa Fluor® 647配合使用。Alexa Fluor® 647 dye 能够被红光最大化激发(e.g. 633 nm He-Ne laser)。此抗体与Alexa Fluor® 647 dye配合使用能激发较强的远红外荧光, 在 665 nm处有吸收峰。

PU.1是转录因子Ets家族中的一员，通过嘌呤富集区域PU-box激活转录目标基因(1)。PU.1在骨髓细胞和淋巴细胞的分化过程中发挥了关键的作用，并在一些骨髓细胞中表达，如 B 淋巴细胞, 巨噬细胞，嗜中性粒细胞，肥大细胞，早期红系细胞和(1,2)。PU.1的浓度对淋巴细胞系的分化和干细胞的分化都有重要的作用(3,4)。另外, PU.1的活性受到磷酸化和造血转录因子互作的影响。PU.1通过CK2在Ser146位点的磷酸化促进了其与IRF4的结合并通过免疫球蛋白 κ 3' 增强子增强了活性(5)。用IL-3处理pro-B细胞导致了PU.1在Ser140位点的磷酸化, 从而增强了PU.1的活性和抗凋亡基因MCL-1的表达(6)。在红系细胞的发育中，GATA1的结合抑制了PU.1活性(7)。过表达PU.1 导致了在Friend病毒入侵时前病毒插入能诱导红白血病 , 而如果降低其表达则与急性的粒细胞性白血病有关(8)。

1. Lloberas, J. et al. (1999) Immunol. Today 20, 184-189.
2. Klemsz, M.J. et al. (1990) Cell 61, 113-124.
3. Dahl, R. and Simon, M.C. (2003) Blood Cells Mol. Dis. 31, 229-233.
4. DeKoter, R.P. and Singh, H. (2000) Science 288, 1439-1441.
5. Pongubala, J.M. et al. (1993) Science 259, 1622-1625.
6. Wang, J.M. et al. (2003) Mol. Cell Biol. 23, 1896-1909.
7. Zhang, P. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8705-8710.
8. Moreau-Gachelin, F. et al. (1998) Nature 331, 277-280.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Pig

Specificity / Sensitivity

PU.1 (9G7) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total PU.1 protein. The antibody does not cross react with other Ets family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PU.1 protein. The antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #2258 reacts with human and mouse PU.1 protein. CST expects that PU.1 (9G7) Rabbit mAb (Alexa Fluor® 647 Conjugate) will also recognize PU.1 in these species.

Background

PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).

1. Lloberas, J. et al. (1999) Immunol. Today 20, 184-189.
2. Klemsz, M.J. et al. (1990) Cell 61, 113-124.
3. Dahl, R. and Simon, M.C. (2003) Blood Cells Mol. Dis. 31, 229-233.
4. DeKoter, R.P. and Singh, H. (2000) Science 288, 1439-1441.
5. Pongubala, J.M. et al. (1993) Science 259, 1622-1625.
6. Wang, J.M. et al. (2003) Mol. Cell Biol. 23, 1896-1909.
7. Zhang, P. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 8705-8710.
8. Moreau-Gachelin, F. et al. (1998) Nature 331, 277-280.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.