Anisomycin

• 产品编号：CST-2222S      品牌：CST       原厂货号：2222S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 激活剂
• 应用分类：

描述：

提供的茴香霉素为冻干粉。对于25 mg/ml浓度贮存液，在10mg冻干粉中加入400 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定，一般是5-50 µg/ml，处理5-60分钟。能溶于DMSO或甲醇。

茴香霉素是一种抗生素，由浅灰链霉菌和玫瑰产色链霉菌产生，能在翻译水平抑制蛋白-蛋白的合成（1）。最近研究发现，它能有力激活应激激酶SAPK/JNK和p38 MAPK以及哺乳动物细胞中的p70/85 S6激酶，导致早期（IE）基因的快速诱导，这些基因包括c-fos, fosB, c-jun, JunB和JunD（1）。研究者已经证明茴香霉素作为一种有效的信号激动剂，能与生长因子和佛波醇酯类一起重复诱导这些IE基因（1,2）。研究还发现茴香霉素能诱导许多癌细胞系的细胞凋亡（3-5）。

原厂资料：

Molecular Characterization

C14H19NO4

Purity

>98%

Directions for Use

Anisomycin is supplied as a lyophilized powder. For a 25 mg/ml stock, reconstitute the 10 mg in 400 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg/ml for 5-60 minutes. Soluble in DMSO or MeOH.

Background

Anisomycin, an antibiotic produced by Streptomyces griseolus and S. roseochromogenes, was originally described to inhibit protein-protein synthesis at the translational level (1). More recently, it is has been well characterized to strongly activate the stress kinases SAPK/JNK and p38 MAPK, as well as p70/85 S6 kinase in mammalian cells, which results in the rapid induction of immediate-early (IE) genes, such as c-fos, fosB, c-jun, JunB, and JunD (1). Investigators have demonstrated that anisomycin acts as a potent signaling agonist, synergizing with growth factors and phorbol esters to superinduce these IE genes (1,2). Research studies have demonstrated that anisomycin induces apoptosis in many cancer cell lines (3-5).

注意事项：

Storage: Store lyophilized or in solution at 4°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 3 months to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles.