Glut4 (1F8) Mouse mAb

• 产品编号：CST-2213S      品牌：CST       原厂货号：2213S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Glut4(1F8)小鼠单克隆抗体识别内源性的Glut4总蛋白水平。它与其它相关蛋白无交叉反应。该单克隆抗体用部分纯化的从大鼠脂肪细胞中获得的包含细胞内葡萄糖转运体的囊泡免疫动物而制成。

一组相关的葡萄糖转运体（GLUT1-5和7）介导哺乳动物非上皮组织中的葡萄糖的易化扩散。在对胰岛素作出反应的组织中，如肌肉和脂肪组织，Glut1有助于基础葡萄糖的摄取，而Glut4负责胰岛素刺激的葡萄糖转运（1-3）。Glut4是一个12次跨膜蛋白，促进葡萄糖按浓度梯度转运。此转运体在未激活的细胞中定位于细胞器（内体），而在胰岛素刺激后转移到细胞表面（1,2,4）。Glut4转位依赖于Akt，其可能磷酸化AS160（一种参与膜转运RabGAP蛋白）（5）。

1. Marette, A. et al. (1992) Am. J. Physiol. 263, C443-C452.
2. Slot, J.W. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7815-7819.
3. James, D.E. et al. (1989) Nature 338, 83-87.
4. Barrett, M.P. et al. (1999) Curr. Opin. Cell Biol. 11, 496-502.
5. Zeigerer, A. et al. (2004) Mol. Biol. Cell 15, 4406-4415.

原厂资料：

Specificity / Sensitivity

Glut4 (1F8) Mouse mAb detects endogenous levels of total Glut4 protein. It does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with partially purified intracellular glucose transporter-containing vesicles obtained from rat adipocytes.

Background

A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Within insulin-responsive tissues such as muscle and fat, Glut1 contributes to basal glucose uptake while Glut4 is responsible for insulin-stimulated glucose transport (1-3). Glut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation (1,2,4). Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking (5).

1. Marette, A. et al. (1992) Am. J. Physiol. 263, C443-C452.
2. Slot, J.W. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7815-7819.
3. James, D.E. et al. (1989) Nature 338, 83-87.
4. Barrett, M.P. et al. (1999) Curr. Opin. Cell Biol. 11, 496-502.
5. Zeigerer, A. et al. (2004) Mol. Biol. Cell 15, 4406-4415.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody