Phospho-S6 Ribosomal Protein (Ser235/236) Antibody

• 产品编号：CST-2211S      品牌：CST       原厂货号：2211S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody检测仅在serine 235和236位点磷酸化的内源性核糖体S6蛋白。该抗体不能检测其它位点磷酸化的核糖体S6蛋白。通过人工合成人源核糖体S6蛋白Ser235和Ser236位点周围序列相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关，而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus

Specificity / Sensitivity

Phospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. This antibody does not detect ribosomal protein S6 phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. Antibodies are purified by protein A and peptide affinity chromatography.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody