Shh (C9C5) Rabbit mAb

• 产品编号：CST-2207S      品牌：CST       原厂货号：2207S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Shh(C9C5)Rabbit mAb 兔单抗识别内源性的总Shh蛋白，此抗体不与转染的IHH或DHH交叉反应。此单抗合成肽段免疫动物产生，该肽段与邻近人源Shh蛋白的Glu53氨基酸残基序列一致。

Hedgehog蛋白（Hh）为分泌信号蛋白，在动物发育中起多种作用，不正常的Hh信号活性可能与众多的出生缺陷有关，失控的Hh通路激活与一些癌症发展有关（1-2）。三种确定的脊椎动物Hh基因是Sonic (Shh), Indian (Ihh)和 Desert (Dhh),所有这些都有各自独特作用，也有重叠作用（3-5）。Hh蛋白被合成为45kDa前体，经过自剪切来产生19kDa氨基末端（Hh-N）和一个C末端肽段（Hh-C）。这氨基末端肽段的C末端变成共价结合于一个胆固醇分子，其N末端乙酰化，这双重修饰Hh-N肽段从细胞释放后参与了已知的所有Hedgehog信号活化过程（6）。

1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
3. Zhang, X.M. et al. (2001) Cell 106, 781-792.
4. Adolphe, C. et al. (2004) Development 131, 5009-5019.
5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Chicken, Xenopus

Specificity / Sensitivity

Shh (C9C5) Rabbit mAb detects the endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu53 of human Shh.

Background

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
3. Zhang, X.M. et al. (2001) Cell 106, 781-792.
4. Adolphe, C. et al. (2004) Development 131, 5009-5019.
5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.