Jagged2 (C83A8) Rabbit mAb

• 产品编号：CST-2205S      品牌：CST       原厂货号：2205S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Jagged2 (C83A8)Rabbit mAb兔源单抗检测内源性的总Jagged2 蛋白，此抗体不与Jagged1交叉反应。此单抗由合成肽段免疫动物产生，该肽段与人Jagged2的邻近1212位脯氨酸的氨基酸残基序列一致。

Notch受体，单次跨膜I 型蛋白，DSL (Delta, Serrate, Lag2)与配体结合激活Notch信号通路。DSL蛋白包括多个EGF-样重复序列和一个DSL结构域，以结合Notch（1,2）。哺乳动物中已经发现有5个DSL蛋白，包括: Jagged1, Jagged2, Delta-like (DLL) 1, 3和4（3）。配体与Notch受体结合导致受体ADAM蛋白酶和γ-分泌酶复合物经两步溶蛋白性裂解。Notch的细胞内区域经释放转入细胞核内激活转录。Notch配体可能通过类似Notch的方式被处理，提示受体-配体相互作用将导致双向的信号传导（4-6）。

1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
3. Chiba, S. (2006) Stem Cells 24, 2437-2447.
4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.

原厂资料：

Specificity / Sensitivity

Jagged2 (C83A8) Rabbit mAb detects endogenous levels of total Jagged2 protein. This antibody does not cross-react with Jagged1.

Jagged2 (C83A8)Rabbit mAb兔源单抗检测内源性的总Jagged2 蛋白，此抗体不与Jagged1交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1212 of human Jagged2.

Background

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).

1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
3. Chiba, S. (2006) Stem Cells 24, 2437-2447.
4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.