Caspase-12 Antibody

• 产品编号：CST-2202P      品牌：Cell Signaling Technology       原厂货号：2202P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Caspase-12 Antibody 抗体能够检测内源水平的全长caspase-12蛋白(55 kDa)以及其剪切后的产物(42 kDa)。此蛋白不与其它的caspases交叉反应。该多克隆抗体是通过合成的小鼠源caspase-12 158氨基酸位点周围残基相应的多肽免疫动物而制备获得。抗体是通过protein A和多肽亲和层析法纯化。

Caspase-12 定位在内质网(ER)上。它很有可能是与ER胁迫诱导的凋亡有关，例如高钙离子浓度、低氧浓度和低氧水平(1,2)。caspase-12激活的机制之一，是与cal pain介导的在T132和K158位点的剪切有关, 这两个位点都在caspase-12的N-末端(2,3)。Caspase-12的另一个潜在的caspase剪切位点在C-末端(3)。在皮层神经元, caspase-12至少部分参与了amyloid-beta 神经毒素的过程中(1)。

1. Nakagawa, T. et al. (2000) Nature 403, 98-103.
2. Nakagawa, T. and Yuan, J. (2000) J. Cell Biol. 150, 887-894.
3. Van de Craen, M. et al. (1997) FEBS Lett. 403, 61-69.

原厂资料：

Specificity / Sensitivity

Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (55 kDa) and its cleaved product (42 kDa). The antibody does not cross-react with other caspases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 158 of mouse caspase-12. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels (1,2). One of the mechanisms for caspase-12 activation is related to calpain mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of the caspase-12 (2,3). Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein (3). In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process (1).

1. Nakagawa, T. et al. (2000) Nature 403, 98-103.
2. Nakagawa, T. and Yuan, J. (2000) J. Cell Biol. 150, 887-894.
3. Van de Craen, M. et al. (1997) FEBS Lett. 403, 61-69.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.