Etoposide

• 产品编号：CST-2200S      品牌：CST       原厂货号：2200S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

提供的依托泊苷为冻干粉。对于50 mM浓度贮存液，在5.9mg冻干粉中加入200 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定，一般是5-50 µM，处理4-24小时。能溶于DMSO（100 mg/ml），难溶于乙醇和水，在水中的最大溶解度约为20-50 µM。

依托泊苷(VP-16)是一种抗肿瘤剂，常用作凋亡诱导物，它还是一种拓扑异构酶II抑制剂（IC50为59.2 µM）（1）。依托泊苷能稳定酶催化性循环中的一种共价的拓扑异构酶II裂解的DNA中间复合物，导致基因组不稳定性和细胞死亡（2,3）。这种作用机制能通过S晚期和G2早期来延误细胞周期的进展（4，5）。

原厂资料：

Molecular Characterization

C29H32O13

Purity

>99%

Directions for Use

Etoposide is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 5.9 mg in 200 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 4-24 hr. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol and water with maximum solubility in water ~20-50 µM.

Background

An anti-tumor agent that is commonly used as an apoptosis inducer, etoposide (VP-16) is a topoisomerase II inhibitor with an IC50 of 59.2 µM (1). Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2,3). This mechanism of action has been shown to delay progression of the cell cycle through the late S and early G2 phase (4,5).

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
In lyophilized form, the chemical is stable for 24 months. Once in
solution, use within 3 months to prevent loss of potency. Aliquot
to avoid multiple freeze/thaw cycles.