Mnk1 (C4C1) Rabbit mAb

• 产品编号：CST-2195S      品牌：CST       原厂货号：2195S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Mnk1 (C4C1) Rabbit mAb兔单抗检测内源性Mnk1总蛋白水平。通过人工合成人源Mnk1蛋白序列的相应多肽去免疫兔子从而制备Mnk1 (C4C1) 兔单抗。

Mitogen-activated protein kinases (MAPKs)可通过不同的细胞外信号激活，并且在调节细胞增殖、分化、生存和凋亡中起着重要的作用(1)。MAPK互作激酶(Mnks或MKNKs)是MAPK通路的直接下游底物，并且首先被Fukunaga和Hunter (2)和Waskiewicz和Cooper (3)分别独自发现。人类中有2个Mnks，分别命名为Mnk1和Mnk2。两个Mnks都具有一个MAPK结合结构域，该功能域能够允许MAPK结合，然后被Erk和p38磷酸化。在Mnks的T-loop位点磷酸化在体外能够刺激它们对底物的活性，如真核生物起始因子4E (eIF4E) (2,3)。eIF4E是蛋白质翻译系统里介导翻译起始的一个重要成分，但是 eIF4E怎样磷酸化去调节翻译起始到目前还在深入研究(4)。

1. Chang, L. and Karin, M. (2001) Nature 410, 37-40.
2. Fukunaga, R. and Hunter, T. (1997) EMBO J. 16, 1921-1933.
3. Waskiewicz, A.J. et al. (1997) EMBO J. 16, 1909-1920.
4. Scheper, G.C. and Proud, C.G. (2002) Eur. J. Biochem. 269, 5350-5359.

原厂资料：

Specificity / Sensitivity

Mnk1 (C4C1) Rabbit mAb detects endogenous levels of total Mnk1 protein.

Source / Purification

Mnk1 (C4C1) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence of human Mnk1.

Background

Mitogen-activated protein kinases (MAPKs) are activated by various extracellular signals and play crucial roles in regulating cell proliferation, differentiation, survival, and apoptosis (1). MAPK-interacting kinases (Mnks or MKNKs) are direct downstream substrates of MAPK and were first discovered independently by the work of Fukunaga and Hunter (2) and Waskiewicz and Cooper (3). There are 2 Mnks in human, termed Mnk1 and Mnk2. Both Mnks possess a MAPK-binding domain that allows them to bind to and then to be phosphorylated by Erk and p38. The phosphorylation in the T-loop of Mnks stimulates their in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF4E) (2,3). eIF4E is a key component of the translational machinery mediating the initiation of translation, but how phosphorylation of eIF4E regulates translation initiation is still under investigation (4).

1. Chang, L. and Karin, M. (2001) Nature 410, 37-40.
2. Fukunaga, R. and Hunter, T. (1997) EMBO J. 16, 1921-1933.
3. Waskiewicz, A.J. et al. (1997) EMBO J. 16, 1909-1920.
4. Scheper, G.C. and Proud, C.G. (2002) Eur. J. Biochem. 269, 5350-5359.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.