MG-132

• 产品编号：CST-2194S      品牌：CST       原厂货号：2194S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

提供的MG-132为冻干粉。对于10 mM浓度贮存液，在1mg冻干粉中加入210.3 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定，一般是5-50 µM，处理1-24小时。能溶于DMSO或乙醇。

MG-132是一种有效的蛋白酶体和钙蛋白酶抑制剂。研究发现MG-132能阻止蛋白酶体底物SucLLVY-MCA（IC50为850nm）和ZLLL-MCA（IC50为100nm）的降解，也能抑制m-需钙蛋白酶（IC50为1.25 µM）的酪蛋白降解活性（1）。研究者已经证明MG-132能抑制TNF-α诱导的NF-κB活化和IL-8在A549细胞中的释放（2）。用MG-132进行的蛋白酶体抑制能阻止短效蛋白的降解，这些短效蛋白与表达增加的HSP和ER伴侣蛋白有关（3）。MG-132能通过一种时间和剂量依赖的方式诱导Hep G2细胞的凋亡（4）。蛋白酶体抑制剂，如MG-132，是研究泛素-蛋白酶体通路的细胞降解的重要工具。

原厂资料：

Molecular Characterization

C26H41N3O5

Purity

>98%

Directions for Use

MG-132 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg in 210.3 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 1-24 hours. Soluble in DMSO or EtOH.

Background

MG-132 is a potent proteasome and calpain inhibitor. Research studies have shown that MG-132 prevents degradation of the proteasome substrates SucLLVY-MCA and ZLLL-MCA with an IC50 of 850 nM and 100 nM, respectively, as well as inhibits casein-degrading activity of m-calpain with an IC50 of 1.25 µM (1). Investigators have demonstrated that MG-132 inhibits TNF-α-induced NF-κB activation and IL-8 release in A549 cells (2). Proteasome inhibition with MG-132 prevents degradation of short-lived proteins, which correlates with increased expression of HSP and ER chaperone proteins (3). MG-132 induces apoptosis in Hep G2 cells in a time- and dose-dependent manner (4). Proteasome inhibitors like MG-132 are important research tools for studying cellular degradation of the ubiquitin-proteasome pathway.

注意事项：

Storage: Store lyophilized or in solution at -20°C, desiccated.
Protect from light. In lyophilized form, the chemical is stable for
24 months. Once in solution, use within 1 month to prevent loss
of potency. Aliquot to avoid multiple freeze/thaw cycles.