Nocodazole

• 产品编号：CST-2190S      品牌：CST       原厂货号：2190S
• 产品分类：生化试剂 > 生物试剂 > 生物活性小分子 > 抑制剂
• 应用分类：

描述：

提供的诺考达唑为冻干粉。对于1mg/ml浓度贮存液，在10 mg冻干粉中加入10 ml DMSO。工作浓度和处理时间根据所需的预期效果来确定，一般是0.1-1 µg/ml，处理12-48小时。能溶于DMSO。诺考达唑是一种抗肿瘤药物，能可逆地干扰微管的聚合（1）。它作为一种细胞周期同步化药物广泛用于细胞生物学实验室，能够诱导有丝分裂的停止，研究者已经证实高浓度的诺考达唑能诱导微管解聚，而低浓度时能改变纺锤体微管动力学，但微管不解聚（2-4）。最近的研究显示诺考达唑是一种常用的多种癌症相关激酶的抑制剂，包括ABL, c-KIT, BRAF, MEK1, MEK2和MET (5)。

原厂资料：

Molecular Characterization

C14H11N3O3S

Purity

>97%

Directions for Use

Nocodazole is supplied as a lyophilized powder. For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µg/ml for 12-48 hr. Soluble in DMSO.

Background

Nocodazole is an anti-neoplastic agent that reversibly interferes with the polymerization of microtubules (1). Widely-used as a cell cycle synchronizing agent in cell biology labs to induce mitotic arrest, investigators have demonstrated that high concentrations of nocodazole induce microtubule depolymerization, whereas low concentrations alter spindle microtubule dynamics, but microtubules do not depolymerize (2-4). Recent research studies have demonstrated nocodazole to be a common inhibitor of various cancer-related kinases, including: ABL, c-KIT, BRAF, MEK1, MEK2, and MET (5).

注意事项：

Storage: Store lyophilized at room temperature or in solution
at -20°C, desiccated. Protect from light. In lyophilized form, the
chemical is stable for 24 months. Once in solution, use within
3 months to prevent loss of potency. Aliquot to avoid multiple
freeze/thaw cycles.