Cyclophilin A Antibody

• 产品编号：CST-2175S      品牌：CST       原厂货号：2175S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cyclophilin A 抗体能够检测内源Cyclophilin A 蛋白总体水平。此多克隆抗体是通过合成对应的Cyclophilin A C-末端周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Cyclophilins 是高度保守的peptidylprolyl 顺式-反式-异构酶 (PPIA)家族成员，是免疫抑制剂药物cyclosporin A (CsA)的靶点(1,2)。Cyclophilin和CsA的复合物能够结合到并抑制钙依赖磷酸酶导致的对转录因子NFAT的抑制和降低细胞因子的产生(3,4)。至于异构酶, cyclophilins可能参与帮助其完成蛋白的折叠。Cyclophilin A能够结合到 HIV的p55 Gag 蛋白上并且是 HIV感染所必需的(5,6)。也有一些证据证明cyclophilins具有核酸酶活性并在凋亡中起了重要的作用(7)。

1. Takahashi, N. et al. (1989) Nature 337, 473-475.
2. Wang, P. and Heitman, J. (2005) Genome Biol. 6, 226.
3. Jain, J. et al. (1993) Nature 365, 352-355.
4. McCaffrey, P.G. et al. (1993) J. Biol. Chem. 268, 3747-3752.
5. Thali, M. et al. (1994) Nature 372, 363-365.
6. Streblow, D.N. et al. (1998) Virology 245, 197-202.
7. Montague, J.W. et al. (1994) J. Biol. Chem. 269, 18877-18880.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog

Specificity / Sensitivity

Cyclophilin A Antibody detects endogenous levels of total Cyclophilin A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Cyclophilin A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cyclophilins are a highly conserved family of peptidylprolyl cis-trans-isomerases (PPIA) that are targets of the immunosuppressant drug cyclosporin A (CsA) (1,2). The complex of cyclophilin and CsA can bind to and inhibit calcineurin which leads to inhibition of the transcription factor NFAT and decreased production of cytokines (3,4). As isomerases, cyclophilins have been proposed to aid in protein folding. Cyclophilin A can bind to the p55 Gag protein of HIV and appears necessary for HIV infection (5,6). There is also some evidence that cyclophilins have nuclease activity and play a role in apoptosis (7).

1. Takahashi, N. et al. (1989) Nature 337, 473-475.
2. Wang, P. and Heitman, J. (2005) Genome Biol. 6, 226.
3. Jain, J. et al. (1993) Nature 365, 352-355.
4. McCaffrey, P.G. et al. (1993) J. Biol. Chem. 268, 3747-3752.
5. Thali, M. et al. (1994) Nature 372, 363-365.
6. Streblow, D.N. et al. (1998) Virology 245, 197-202.
7. Montague, J.W. et al. (1994) J. Biol. Chem. 269, 18877-18880.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.