Rab4 Antibody

• 产品编号：CST-2167P      品牌：Cell Signaling Technology       原厂货号：2167P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab4 Antibody识别内源性的Rab4总蛋白。该多克隆抗体用与人类Rab4中Asp128位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。Rab4是小Rab GTP酶Ras超家族的成员，与内吞作用有关。Rab4定位于早期内体/回收内体上，是分选和回收膜和蛋白质的的关键调节者（1,2）。Rab4有两种形式，Rab4A和Rab4B，两种形式都定位于相似的细胞区域，并且被认为具有相似的功能（4）。Rab4与数个Rab4效应物以复合物在特定的内体位点相互作用，促进膜/蛋白循环（1,3）。

1. Zerial, M. and McBride, H. (2001) Nat. Rev. Mol. Cell Biol. 2, 107-117.
2. van der Sluijs, P. et al. (1992) Cell 70, 729-740.
3. Deneka, M. et al. (2003) EMBO J. 22, 2645-2657.
4. Krawczyk, M. et al. (2007) Nucleic Acids Res. 35, 595-605.

原厂资料：

Specificity / Sensitivity

Rab4 Antibody detects endogenous levels of total Rab4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp128 of human Rab4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Rab4 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis. Rab4 is localized at early endosomes/recycling endosomes and functions as a key regulator for sorting/recycling of membrane and proteins (1,2). Rab4 has two isoforms, Rab4A and Rab4B, both of which are localized in similar cellular compartments and are believed to have similar functions (4). Rab4 interacts with several Rab4 effectors in a complex on a special endosome site that promotes membrane/protein recycling (1,3).

1. Zerial, M. and McBride, H. (2001) Nat. Rev. Mol. Cell Biol. 2, 107-117.
2. van der Sluijs, P. et al. (1992) Cell 70, 729-740.
3. Deneka, M. et al. (2003) EMBO J. 22, 2645-2657.
4. Krawczyk, M. et al. (2007) Nucleic Acids Res. 35, 595-605.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.