NSF Antibody

• 产品编号：CST-2145S      品牌：CST       原厂货号：2145S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NSF Antibody识别内源性的NSF总蛋白。该多克隆抗体用与人类NSF蛋白中Leu524位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

若干蛋白间相互作用对于胞吞时膜融合至关重要。膜融合需要与膜供体和受体结合的SNARE1蛋白间的相互作用（1,2）。膜融合后，α-SNAP胞浆接头蛋白结合到SNARE复合物。n-乙基马来酰亚胺因子-敏感（NSF），是一个六聚ATPase，可以结合到α-SNAP/SNARE复合体介导SNARE在膜融合时解聚（3,4）。NSF的ATP酶活性诱导α-SNAP/SNARE复合物构象改变，导致其从膜上脱离、膜融合以及SNARE复合物最终再循环以备后续膜融合使用（3,4）。

1. Ungermann, C. and Langosch, D. (2005) J Cell Sci 118, 3819-28.
2. Leabu, M. (2006) J Cell Mol Med 10, 423-7.
3. May, A.P. et al. (2001) J Biol Chem 276, 21991-4.
4. Dalal, S. et al. (2004) Mol Biol Cell 15, 637-48.

原厂资料：

Specificity / Sensitivity

NSF Antibody detects endogenous levels of total NSF protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu524 of human NSF. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).

1. Ungermann, C. and Langosch, D. (2005) J Cell Sci 118, 3819-28.
2. Leabu, M. (2006) J Cell Mol Med 10, 423-7.
3. May, A.P. et al. (2001) J Biol Chem 276, 21991-4.
4. Dalal, S. et al. (2004) Mol Biol Cell 15, 637-48.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody.