Rab5 Antibody

• 产品编号：CST-2143P      品牌：Cell Signaling Technology       原厂货号：2143P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab5 Antibody识别内源性的Rab5总蛋白水平。该多克隆抗体用与人类Rab5中Gly190位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。Rab5是小Rab GTP酶Ras超家族的成员。Rab5定位于细胞膜和早期内体，以早期内吞时囊泡转运的关键调节者而发挥作用（1）。Rab5在GTP/GDP状态下的构象改变对其生物学功能至关重要，因为其是内吞作用多个步骤中的一个速率限制调节者（1,2）。Rab5与数个Rab5特异性效应物相互作用发挥作用（1-3）。这些蛋白在内体Rab区域上与Rab5形成的蛋白复合物，促进Rab5-运送靶标在内吞体和细胞膜的转运循环。

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
3. Haas, A.K. et al. (2005) Nat Cell Biol 7, 887-93.

原厂资料：

Specificity / Sensitivity

Rab5 Antibody detects endogenous levels of total Rab5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Gly190 of human Rab5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (1). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (1,2). Rab5 exerts its function by interacting with several Rab5-specific effectors (1-3). These proteins form complexes with Rab5 on a specialized Rab domain of the endosome and promote recycling of Rab5-cargo targets between endosome and the plasma membrane.

1. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
2. van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
3. Haas, A.K. et al. (2005) Nat Cell Biol 7, 887-93.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.