LSD1 Antibody

• 产品编号：CST-2139S      品牌：CST       原厂货号：2139S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LSD1 Antibody能够检测内源性LSD1总蛋白水平。通过合成的与人源LSD1蛋白氨基端相应多肽片段去免疫动物从而制备此多克隆抗体。通过蛋白蛋白A和多肽亲和层析纯化获得。

Lysine-specific demethylase 1 (LSD1; 也称为AOF2和BHC110)是一个核胺氧化酶同系物，它能作为一个组蛋白去甲基化和转录辅因子(1)。明显的组蛋白赖氨酸残基的甲基化水平能够特异性调节基因激活和抑制。例如，histone H3蛋白Lys4位点的甲基化通过协调BPTF和WDR5的招募有助于转录激活，而BPTF是NURF染色质重塑复合物的成分，并且WDR5是多种组蛋白甲基转移酶复合物成分(2,3)。与此相反，histone H3蛋白Lys9位点甲基化通过招募HP1有助于转录抑制(4,5)。LSD1是CoREST转录辅助抑制因子复合物的成分，该复合物也包含CoREST、CtBP、HDAC1和HDAC2。作为这个复合物的一部分，在非神经元细胞中LSD1使单甲基化和双甲基化的histone H3蛋白Lys4位点去甲基化，这通过一个FAD依赖的氧化反应去有助于神经特异性基因的抑制(1,6,7)。相反，在人源前列腺细胞中LSD1与雄激素受体有关联，从而使单甲基化和双甲基化histone H3蛋白Lys9位点去甲基化，以及有助于雄激素受体依赖的转录激活(8)。依据基因背景，LSD1的功能既可以是转录的辅助抑制因子，又可以使辅助激活因子。LSD1活性通过胺氧化酶抑制剂pargyline、deprenyl、clorgyline和tranylcypromine被抑制(8)。

1. Shi, Y. et al. (2004) Cell 119, 941-953.
2. Wysocka, J. et al. (2006) Nature 442, 86-90.
3. Wysocka, J. et al. (2005) Cell 121, 859-872.
4. Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295, 2080-2083.
5. Nielsen, P.R. et al. (2002) Nature 416, 103-107.
6. Shi, Y.J. et al. (2005) Mol. Cell 19, 857-864.
7. Lee, M.G. et al. (2005) Nature 437, 432-435.
8. Metzger, E. et al. (2005) Nature 437, 436-439.

原厂资料：

Specificity / Sensitivity

LSD1 Antibody detects endogenous levels of total LSD1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).

1. Shi, Y. et al. (2004) Cell 119, 941-953.
2. Wysocka, J. et al. (2006) Nature 442, 86-90.
3. Wysocka, J. et al. (2005) Cell 121, 859-872.
4. Jacobs, S.A. and Khorasanizadeh, S. (2002) Science 295, 2080-2083.
5. Nielsen, P.R. et al. (2002) Nature 416, 103-107.
6. Shi, Y.J. et al. (2005) Mol. Cell 19, 857-864.
7. Lee, M.G. et al. (2005) Nature 437, 432-435.
8. Metzger, E. et al. (2005) Nature 437, 436-439.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.