Phospho-Rev-erbα (Ser55/59) Antibody

• 产品编号：CST-2129S      品牌：CST       原厂货号：2129S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

当丝氨酸（55/59位）磷酸化时，Phospho-Rev-erbα (Ser55/59) Antibody能够检测转染水平的Rev-erbα蛋白。该抗体不与其他核受体蛋白包括Rev-erbβ，发生交叉反应。该多克隆抗体通过用合成磷酸肽免疫动物制备，该合成磷酸肽是人Rev-erbα丝氨酸（55和59位）附近的残基。抗体是由蛋白A和肽亲和层析纯化。

反转定向c-erbA基因α（Rev-erbα, EAR-1或 NR1D1）是一种广泛表达的孤儿核受体蛋白家族成员（1）。Rev-erbα在脂肪组织、骨骼肌、脑和肝中高表达，并调节细胞的增殖和分化。在脂肪细胞分化过程中表达增加，REV-erbα异位表达会增强3T3-L1细胞向脂肪细胞分化（2）。此外，REV-erbα在肝细胞内按昼夜节律振荡表达，并调控BMAL1、ApoA-I和ApoC-III，所有关键调节器都是昼夜节律表达（3,4,5,6,7）。由GSK3β磷酸化REV-erbα的丝氨酸（55和59位），表现出稳定的REV-erbα蛋白质水平，在生物钟的同步和维持中很重要（8）。同样，REV-erbα针对NF-κB应答基因IL-6和COX-2调节炎症（9）。 REV-erbα缺乏激活功能2结构域，这对核受体其他家族成员的配体依赖型转录激活是需要的；因此它作为阻遏蛋白起作用，招募核受体辅阻遏物（N-COR）/ HDAC3复合物的靶基因，抑制转录（10）。

1. Harding, H.P. and Lazar, M.A. (1993) Mol Cell Biol 13, 3113-21.
2. Chawla, A. and Lazar, M.A. (1993) J Biol Chem 268, 16265-9.
3. Torra, I.P. et al. (2000) Endocrinology 141, 3799-806.
4. Preitner, N. et al. (2002) Cell 110, 251-60.
5. Vu-Dac, N. et al. (1998) J Biol Chem 273, 25713-20.
6. Coste, H. and Rodríguez, J.C. (2002) J Biol Chem 277, 27120-9.
7. Raspé, E. et al. (2002) J Lipid Res 43, 2172-9.
8. Yin, L. et al. (2006) Science 311, 1002-5.
9. Migita, H. et al. (2004) FEBS Lett 561, 69-74.
10. Yin, L. and Lazar, M.A. (2005) Mol Endocrinol 19, 1452-9.

原厂资料：

Specificity / Sensitivity

Phospho-Rev-erbα (Ser55/59) Antibody detects transfected levels of Rev-erbα protein when phosphorylated at Ser55/59. The antibody does not cross-react with other nuclear receptor proteins, including Rev-erbβ.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser55 and Ser59 of human Rev-erbα. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3,4,5,6,7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10).

1. Harding, H.P. and Lazar, M.A. (1993) Mol Cell Biol 13, 3113-21.
2. Chawla, A. and Lazar, M.A. (1993) J Biol Chem 268, 16265-9.
3. Torra, I.P. et al. (2000) Endocrinology 141, 3799-806.
4. Preitner, N. et al. (2002) Cell 110, 251-60.
5. Vu-Dac, N. et al. (1998) J Biol Chem 273, 25713-20.
6. Coste, H. and Rodríguez, J.C. (2002) J Biol Chem 277, 27120-9.
7. Raspé, E. et al. (2002) J Lipid Res 43, 2172-9.
8. Yin, L. et al. (2006) Science 311, 1002-5.
9. Migita, H. et al. (2004) FEBS Lett 561, 69-74.
10. Yin, L. and Lazar, M.A. (2005) Mol Endocrinol 19, 1452-9.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.