Alpha-Fodrin Antibody

• 产品编号：CST-2122S      品牌：CST       原厂货号：2122S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Alpha-Fodrin Antibody 能够检测内源性水平全长α-胞衬蛋白(240 kDa)和在Asp1185处剪切的大片段(150 kDa)。该抗体不识别全长α-胞衬蛋白。该多克隆抗体是采用合成的人源α-胞衬蛋白 Asp1185 周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

odrin 胞衬蛋白（来自非红细胞，但与血影蛋白类似）是普遍表达的膜相关的细胞骨架蛋白，由α-和β-亚基组成（1）。这种蛋白质对于维持正常的膜结构和支持细胞表面蛋白的功能是很重要的（1）。α-胞衬蛋白在细胞凋亡过程中是半胱天冬酶裂解的主要靶点之一。这个全长240 kDa的蛋白可以在几个位点内被激活的半胱天冬酶裂解，产生150 kDa的氨基末端，120 kDa的羧基末端和35 kDa的主要产物（2-5）。α-胞衬蛋白的裂解导致膜故障和细胞收缩。

1. Bennett, V. and Gilligan, D.M. (1993) Annu. Rev. Cell Biol. 9, 27-66.
2. Vanags, D. M. et al. (1996) J. Biol. Chem. 271, 31075-31085.
3. Cryns, V. L. et al. (1996) J. Biol. Chem. 271, 31277-31282.
4. Wang, K. K. et al. (1998) J. Biol. Chem. 273, 22490-22497.
5. Janicke, R. U. et al. (1998) J. Biol. Chem. 273, 15540-15545.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken

Specificity / Sensitivity

Alpha-Fodrin Antibody detects endogenous levels of full length alpha-fodrin (240 kDa) and its large fragment (150 kDa) resulting from cleavage at aspartic acid 1185.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1185 of human alpha-fodrin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha- and beta-subunits (1). This protein is important for maintaining normal membrane structure and supporting cell surface protein function (1). Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases to yield amino-terminal 150 kDa, carboxy-terminal 120 kDa and 35 kDa major products (2-5). Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.

1. Bennett, V. and Gilligan, D.M. (1993) Annu. Rev. Cell Biol. 9, 27-66.
2. Vanags, D. M. et al. (1996) J. Biol. Chem. 271, 31075-31085.
3. Cryns, V. L. et al. (1996) J. Biol. Chem. 271, 31277-31282.
4. Wang, K. K. et al. (1998) J. Biol. Chem. 273, 22490-22497.
5. Janicke, R. U. et al. (1998) J. Biol. Chem. 273, 15540-15545.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.