FABP4 Antibody

• 产品编号：CST-2120S      品牌：CST       原厂货号：2120S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FABP4抗体识别内源性的FABP4总蛋白水平。该多克隆抗体用与小鼠FABP4氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白亲和层析纯化而得。

脂肪酸结合蛋白（FABPs）结合脂肪酸和其他脂质，功能类似细胞质内的脂质伴侣（1）。它们参与脂肪酸和其他脂质的运输以及各种细胞的信号通路（2）。在脂肪细胞中发现的主要的脂肪酸结合蛋白是FABP4，也称为脂肪细胞脂肪酸结合蛋白或aP2。FABP4也表达于巨噬细胞（3）。FABP4敲除的小鼠以高脂和高卡路里饮食喂养后发生肥胖，但并不发生胰岛素抵抗或者糖尿病，这表明此蛋白可能与肥胖、胰岛素抵抗和糖尿病有关（4）。缺乏FABP4和ApoE的小鼠与仅缺乏ApoE的小鼠相比显示出对动脉粥样硬化的抵抗（3）。携带FABP4突变的小鼠显示出FABP4的表达减少并且更倾向于发生2型糖尿病和冠心病。人类的相关研究显示了类似的模式，这表明FABP4可能成为治疗这些疾病潜在的靶点（1）。

1. Tuncman, G. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 6970-6975.
2. Haunerland, N.H. and Spener, F. (2004) Prog. Lipid Res. 43, 328-349.
3. Makowski, L. et al. (2001) Nat. Med. 7, 699-705.
4. Hotamisligil, G.S. et al. (1996) Science 274, 1377-1379.

原厂资料：

Specificity / Sensitivity

FABP4 Antibody detects endogenous levels of total FABP4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse FABP4. Antibodies are purified by peptide affinity chromatography.

Background

Fatty acid binding proteins (FABPs) bind to fatty acids and other lipids to function as cytoplasmic lipid chaperones (1). They participate in the transport of fatty acids and other lipids to various cellular pathways (2). The predominant fatty acid binding protein found in adipocytes is FABP4, also called adipocyte fatty acid binding protein or aP2. FABP4 is also expressed in macrophages (3). FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes (4). Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE (3). Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders (1).

1. Tuncman, G. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 6970-6975.
2. Haunerland, N.H. and Spener, F. (2004) Prog. Lipid Res. 43, 328-349.
3. Makowski, L. et al. (2001) Nat. Med. 7, 699-705.
4. Hotamisligil, G.S. et al. (1996) Science 274, 1377-1379.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody