Hexokinase II Antibody

• 产品编号：CST-2106S      品牌：CST       原厂货号：2106S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

己糖激酶II抗体识别内源性的全部己糖激酶II蛋白。该多克隆抗体用与人thioredoxin II氨基酸序列对应的人工合成肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

己糖激酶催化糖酵解第一步中葡萄糖到6-磷酸葡萄糖的转化（1）。目前已确定哺乳动物己糖激酶的四个不同的亚型，分别为I, II, III, IV（葡萄糖激酶）。己糖激酶I、II、III与线粒体外膜相关联，在癌细胞保持高效率无氧酵解（Warburg效应）中至关重要（1），这补偿了快速生长增殖的癌细胞的能量需求（2,3）。

1. WARBURG, O. (1956) Science 123, 309-14.
2. Semenza, G.L. (2000) Crit Rev Biochem Mol Biol 35, 71-103.
3. Smith, T.A. (2000) Br J Biomed Sci 57, 170-8.

原厂资料：

Specificity / Sensitivity

Hexokinase II Antibody detects endogenous levels of total hexokinase (isoform II) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase (isoform II). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

1. WARBURG, O. (1956) Science 123, 309-14.
2. Semenza, G.L. (2000) Crit Rev Biochem Mol Biol 35, 71-103.
3. Smith, T.A. (2000) Br J Biomed Sci 57, 170-8.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.