Non-phospho-Src (Tyr416) (7G9) Mouse mAb

• 产品编号：CST-2102S      品牌：CST       原厂货号：2102S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Non-phospho-Src (Tyr416) (7G9) Mouse mAb鼠单抗能够检测内源性Tyr416位点非磷酸化的Src蛋白水平。该抗体能与其他相同位点去磷酸化的Src家族成员(Lyn, Fyn, Lck, Yes and Hck) 发生交叉反应。该单克隆抗体是采用合成的与人源Src蛋白Tyr416位点周围残基相一致的合成肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。

Src家族蛋白酪氨酸激酶包括Src, Lyn, Fyn, Yes, Lck, Blk, Hck, 对调节真核细胞的生长和分化很重要(1)。Src的活性是通过两个酪氨酸位点的磷酸化来调控的，但是这两者的调控效应相反。激酶结构域的活化环中的Tyr416位点的磷酸化，能够上调酶活性；而蛋白羧基端尾部Tyr527位点被Csk磷酸化后，则会使酶的活性降低(2)。

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus

Specificity / Sensitivity

Non-phospho-Src (Tyr416) (7G9) Mouse mAb detects endogenous levels of Src only when dephosphorylated at tyrosine 416. The antibody cross-reacts with other Src family members (Lyn, Fyn, Lck, Yes and Hck) dephosphorylated at equivalent sites.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr416 of human Src.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody