SCF (C19H6) Rabbit mAb

• 产品编号：CST-2093S      品牌：CST       原厂货号：2093S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SCF (C19H6)Rabbit mAb兔单抗能识别免疫组化染色和流式细胞仪实验中的内源性SCF蛋白，western blotting 实验中识别重组SCF蛋白.单克隆抗体由合成肽段免疫动物产生，该肽段与人SCF的氨基末端氨基酸残基序列一致。

干细胞因子（SCF）是一类对造血作用、黑素生成和生育必需的生长因子，SCF也被认为是肥大细胞生长因子（MCGF），Steel因子（SLF）,或盒式配体(KL) (（1-3）。SCF通过结合并激活c-kit调发挥其的生物学功能（4）。SCF在胞质蛋白酪氨酸激酶结构域反式自磷酸化后诱导c-kit二聚化， 导致随后信号蛋白的招募，酪氨酸底物的磷酸化，以及多个信号通路的激活（5,6）。SCF/c-Kit可能参与人恶性肿瘤的生长控制（7）.

1. Smith, M.A. et al. (2001) Blood Rev. 15, 191-197.
2. Linnekin, D. (1999) Int. J. Biochem. Cell Biol. 31, 1053-1074.
3. Huang, E. et al. (1990) Cell 63, 225-233.
4. Jiang, X. et al. (2000) EMBO J. 19, 3192-3203.
5. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31, 1037-1051.
6. Ueda, S. et al. (2002) Blood 99, 3342-3349.
7. Attoub, S. et al. (2002) Cancer Res. 62, 4879-4883.

原厂资料：

Specificity / Sensitivity

SCF (C19H6) Rabbit mAb detects endogenous levels of SCF by immunohistochemical staining and by flow cytometry. Western blotting detects recombinant SCF.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids in the N-terminal sequence of human SCF.

Background

Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis and fertility. SCF is also known as mast cell growth factor (MCGF), steel factor (SLF), or kit ligand (KL) (1-3). SCF mediates its biological effects by binding to and activating c-Kit (4). SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates and activation of multiple signaling pathways (5,6). SCF/c-Kit may take part in the growth control of human malignancies (7).

1. Smith, M.A. et al. (2001) Blood Rev. 15, 191-197.
2. Linnekin, D. (1999) Int. J. Biochem. Cell Biol. 31, 1053-1074.
3. Huang, E. et al. (1990) Cell 63, 225-233.
4. Jiang, X. et al. (2000) EMBO J. 19, 3192-3203.
5. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31, 1037-1051.
6. Ueda, S. et al. (2002) Blood 99, 3342-3349.
7. Attoub, S. et al. (2002) Cancer Res. 62, 4879-4883.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.