Centrin-2 Antibody

• 产品编号：CST-2091S      品牌：CST       原厂货号：2091S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Centrin-2 Antibody检测内源性centrin-2总蛋白。通过人工合成人源centrin-2蛋白氨基末端周围相应的多肽片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

在真核细胞中，Centrins蛋白是小的保守微管成核蛋白质，其定位于中心体，又称为微管组织中心（microtubule organizing centre,MTOC）。Centrin-1 蛋白与含有纤毛和鞭毛的细胞有联系，然而 centrin-2 和-3普遍表达，并且在细胞分裂时中心体复制有着重要作用，和MTOC的结构和功能一样(1-3)。研究已经显示人源centrin-2蛋白定位于核孔，并且在mRNA输出的调节中有作用 (4)。centrin-2蛋白的酵母同系物CDC31蛋白参与控制蛋白质降解和敏感DNA损伤 (5)。

1. Salisbury, J.L. (2007) J Cell Physiol 213, 420-8.
2. Salisbury, J.L. et al. (2002) Curr Biol 12, 1287-92.
3. Middendorp, S. et al. (2000) J Cell Biol 148, 405-16.
4. Resendes, K.K. et al. (2008) Mol Cell Biol 28, 1755-69.
5. Chen, L. and Madura, K. (2008) Mol Cell Biol 28, 1829-40.

原厂资料：

Specificity / Sensitivity

Centrin-2 Antibody detects endogenous levels of total centrin-2 protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human centrin-2. Antibodies are purified using protein A and peptide affinity chromatography. 

Background

Centrins are small conserved microtubule nucleating proteins localized to centrosomes, also known as microtubule organizing centers (MTOC), in eukaryotic cells. Centrin-1 is associated with cells that have cilia and flagella, whereas centrin-2 and -3 are ubiquitously expressed and important in centrosome duplication during cell division, as well as the structure and function of the MTOC (1-3). Human centrin-2 has also been shown to localize to nuclear pores and to have a role in regulation of mRNA export (4). The yeast ortholog of centrin-2, CDC31, plays a part in control of protein degradation and sensitivity to DNA damage (5).

1. Salisbury, J.L. (2007) J Cell Physiol 213, 420-8.
2. Salisbury, J.L. et al. (2002) Curr Biol 12, 1287-92.
3. Middendorp, S. et al. (2000) J Cell Biol 148, 405-16.
4. Resendes, K.K. et al. (2008) Mol Cell Biol 28, 1755-69.
5. Chen, L. and Madura, K. (2008) Mol Cell Biol 28, 1829-40.

注意事项：

For Research Use Only. Not For Use In Diagnostic Procedures.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.